Marco Y, Rochaix J D
Mol Gen Genet. 1980;177(4):715-23. doi: 10.1007/BF00272684.
Hybridization of cytoplasmic ribosomal RNA (rRNA) to restriction endonuclease digests of nuclear DNA of Chlamydomonas reinhardii reveals two BamHI ribosomal fragments of 2.95 and 2.35 x 10(6) d and two SalI ribosomal fragments of 3.8 and 1.5 x 10(6) d. The ribosomal DNA (rDNA) units 5.3 x 10(6) d in size, appear to be homogeneous since no hybridization of rDNA to other nuclear DNA fragments can be detected. The two BamHI and SalI ribosomal fragments have been cloned and a restriction map of the ribosomal unit has been established. The location of the 25S, 18S and 5.8S rRNA genes has been determined by hybridizing the rRNAs to digests of the ribosomal fragments and by observing RNA/DNA duplexes in the electron microscope. The data also indicate that the rDNA units are arranged in tandem arrays. The 5S rRNA genes are not closely located to the 25S and 18S rRNA genes since they are not contained within the nuclear rDNA unit. In addition no sequence homology is detectable between the nuclear and chloroplast rDNA units of C. reinhardii.
莱茵衣藻细胞质核糖体RNA（rRNA）与核DNA的限制性内切酶消化产物杂交，显示出两个大小分别为2.95和2.35×10⁶道尔顿的BamHI核糖体片段，以及两个大小分别为3.8和1.5×10⁶道尔顿的SalI核糖体片段。大小为5.3×10⁶道尔顿的核糖体DNA（rDNA）单位似乎是同质的，因为未检测到rDNA与其他核DNA片段的杂交。已克隆了两个BamHI和SalI核糖体片段，并建立了核糖体单位的限制性图谱。通过将rRNA与核糖体片段的消化产物杂交，并在电子显微镜下观察RNA/DNA双链体，确定了25S、18S和5.8S rRNA基因的位置。数据还表明，rDNA单位排列成串联阵列。5S rRNA基因与25S和18S rRNA基因的位置不紧密，因为它们不包含在核rDNA单位内。此外，在莱茵衣藻的核rDNA单位和叶绿体rDNA单位之间未检测到序列同源性。
