Malnoë P, Rochaix J D
Mol Gen Genet. 1978 Nov 9;166(3):269-75. doi: 10.1007/BF00267618.
The genes coding for 4S RNA have been localized on the physical map of the chloroplast genome of Chlamydomonas reinhardii by hybridizing 32P-labelled 4S RNA to EcoRI, BamHI, Bg1II and Hind III chloroplast DNA digests and to hybrid plasmids containing EcoRI and Bam HI chloroplast DNA fragments. At least 10 EcoRI and 7 Bam HI fragments carry sequences coding for 4S RNA. These genes are interspersed throughout the genome. The spacer between the 16S and 23S ribosomal RNA genes, which is repeated twice per chloroplast DNA molecule, codes for at least one 4S RNA, shown to be transcribed from the same strand as the ribosomal RNAs.
通过将用³²P标记的4S RNA与莱茵衣藻叶绿体DNA经EcoRI、BamHI、Bg1II和Hind III酶切后的片段以及含有EcoRI和Bam HI叶绿体DNA片段的杂交质粒进行杂交，编码4S RNA的基因已定位在莱茵衣藻叶绿体基因组的物理图谱上。至少10个EcoRI片段和7个Bam HI片段携带编码4S RNA的序列。这些基因散布于整个基因组中。16S和23S核糖体RNA基因之间的间隔区，每个叶绿体DNA分子重复两次，至少编码一种4S RNA，已证明它与核糖体RNA转录自同一条链。
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