Waters L C, Mullin B C, Bailiff E G, Popp R A
Biochim Biophys Acta. 1980 Jun 27;608(1):112-26. doi: 10.1016/0005-2787(80)90139-2.
The tRNAs that are bound to the genomic RNAs of several murine, feline, and primate retroviruses have been identified. Transfer RNAs were divided into those loosely bound and those tightly bound by stepwise thermal dissociation of the 70 S RNA. They were then identified and semiquantitated by aminoacylation. Proline tRNA is the most tenaciously bound tRNA in several strains of murine leukemia virus, two strains of feline leukemia virus, and the primate viruses simian sarcoma, baboon endogenous, and gibbon ape lymphoma. In the feline xenotropic virus, RD-114, tRNAGly is enriched in the most tightly bound fraction. In Mason-Pfizer monkey virus, as in the murine mammary tumor virus, tRNALys is the tRNA most tenaciously bound to its genomic RNA. Besides the most tightly associated tRNA, one or more different tRNAs are found in relatively large amounts in association with the 70 S RNA. (For convenience, we refer to the largest RNA ccomplex (50-70 S) isolated from any of the retroviruses studies as '70 S' RNA.) These tRNAs can be distinguished from the most tightly bound tRNA by the fact that they can be dissociated at lower temperatures. However, they occur in the same relative abundance as the tightly bound tRNA.
几种鼠类、猫科和灵长类逆转录病毒的基因组RNA所结合的tRNA已被鉴定出来。通过70S RNA的逐步热解离，将转运RNA分为松散结合型和紧密结合型。然后通过氨酰化作用对它们进行鉴定和半定量。在几种鼠白血病病毒株、两种猫白血病病毒株以及灵长类病毒猿猴肉瘤病毒、狒狒内源性病毒和长臂猿淋巴瘤病毒中，脯氨酸tRNA是结合最紧密的tRNA。在猫嗜异性病毒RD - 114中，甘氨酰tRNA在结合最紧密的部分富集。在梅森 - Pfizer猴病毒中，与鼠乳腺肿瘤病毒一样，赖氨酰tRNA是与其基因组RNA结合最紧密的tRNA。除了结合最紧密的tRNA外，还发现一种或多种不同的tRNA以相对大量的形式与70S RNA结合。（为方便起见，我们将从任何一种所研究的逆转录病毒中分离出的最大RNA复合物（50 - 70S）称为“70S”RNA。）这些tRNA与结合最紧密的tRNA的区别在于它们能在较低温度下解离。然而，它们与紧密结合的tRNA的相对丰度相同。
