McGill M
Cytogenet Cell Genet. 1980;26(2-4):117-26. doi: 10.1159/000131433.
Small lymphocytes differentiate into functionally active blast cells in vitro upon stimulation with such mitogens as phytohemagglutinin and sodium periodate. If stimulated lymphocytes are subsequently treated with the nucleic acid intercalating dye ethidium bromide, electron-dense complexes containing nucleic acid are formed in mitochondria, protein synthesis in mitochondria is inhibited, and lymphoblast division ceases. Formation of complexes and the development of morphologically abnormal mitochondria provide ultrastructural evidence of mitochondrial protein inhibition and serve as markers for mitogen-responsive lymphocytes. The formation of these abnormalities in all mitochondria of treated megakaryocytes and 22% of mitochondria in platelets indicates that platelets contain functional nucleic acid and that the induced structural changes may be occurring in a less-differentiated (i.e., younger) subpopulation of circulating platelets.
小淋巴细胞在受到诸如植物血凝素和高碘酸钠等促细胞分裂剂刺激后，可在体外分化为功能活跃的母细胞。如果随后用核酸嵌入染料溴化乙锭处理受刺激的淋巴细胞，线粒体中会形成含核酸的电子致密复合物，线粒体中的蛋白质合成受到抑制，淋巴母细胞分裂停止。复合物的形成以及形态异常的线粒体的出现，为线粒体蛋白抑制提供了超微结构证据，并可作为促细胞分裂剂反应性淋巴细胞的标志物。在处理过的巨核细胞的所有线粒体以及血小板中22%的线粒体中出现这些异常，表明血小板含有功能性核酸，并且诱导的结构变化可能发生在循环血小板中分化程度较低（即较年轻）的亚群中。
