Hess A
J Histochem Cytochem. 1978 Feb;26(2):141-4. doi: 10.1177/26.2.624834.
The difference between dopamine and noradrenaline after ordinary histofluorescent procedures cannot be discerned. Reserpine treatment results in depletion of fluorescent material from dopaminergic and noradrenergic peripheral nervous structures. Administration of reserpine, 1 mg/kg subcutaneously for 3 hr, followed by intraperitoneal injection of 200 mg/kg levodopa methyl ester on 0.9% saline for 90 min, result in refluorescence of dopaminergic (glomus cells of the carotid body) but not noradrenergic (sympathetic ganglion cells, nerves of atrial heart muscle and blood vessels) structures. Hence, the sequential administration of these readily available drugs and the application of ordinary histofluorescent techniques result in a simple procedure for distinguishing dopamine from noradrenaline in the fluorescence microscope.
普通组织荧光法无法区分多巴胺和去甲肾上腺素。利血平处理会导致多巴胺能和去甲肾上腺素能外周神经结构中的荧光物质耗竭。皮下注射1mg/kg利血平3小时，随后腹腔注射200mg/kg左旋多巴甲酯于0.9%生理盐水中90分钟，结果显示多巴胺能结构（颈动脉体的球细胞）出现再荧光，而去甲肾上腺素能结构（交感神经节细胞、心房心肌和血管神经）则未出现。因此，依次给予这些易于获取的药物并应用普通组织荧光技术，可在荧光显微镜下形成一种区分多巴胺和去甲肾上腺素的简单方法。
