Nusse R, Michalides R, Boot L M, Röpcke G
Int J Cancer. 1980 Mar 15;25(3):377-83. doi: 10.1002/ijc.2910250312.
The expression of the mouse mammary tumor virus (MMTV) in hormone-induced mammary tumors was investigated by means of a radioimmunoassay for two major MMTV proteins, gp52 and p27. MMTV proteins were isolated on lectin affinity- and ion-exchange chromatography columns. The purified viral proteins were electrophoretically homogeneous and retained immunoreactivity after labelling with 125iodine. Standard competition assays showed that group-specific antigenic determinants were reacting. Mammary tumors were induced in three strains of mice with a low natural incidence of mammary tumors, C57BL, O20 and C3Hf, by a combined hormone treatment, consisting of hypophyseal isografts and administration of progesterone and estrone. Mammary tumors and mammary glands of hormone-treated animals were extracted and used for competition radioimmunoassays. In general, the tumorigenic hormone treatment resulted in enhanced amounts of MMTV proteins in the mammary glands, compared to the amounts found in lactating mammary glands of untreated animals. The levels of MMTV proteins in the mammary tumors were lower than in the mammary glands.
通过针对小鼠乳腺肿瘤病毒（MMTV）的两种主要蛋白gp52和p27的放射免疫测定法，研究了激素诱导的乳腺肿瘤中MMTV的表达情况。MMTV蛋白通过凝集素亲和层析柱和离子交换层析柱进行分离。纯化后的病毒蛋白在电泳上是均一的，并且在用125碘标记后仍保留免疫反应性。标准竞争试验表明，组特异性抗原决定簇发生了反应。通过联合激素处理，即垂体同基因移植以及给予孕酮和雌酮，在乳腺肿瘤自然发生率较低的三种小鼠品系C57BL、O20和C3Hf中诱导出乳腺肿瘤。对接受激素处理的动物的乳腺肿瘤和乳腺进行提取，并用于竞争放射免疫测定。总体而言，与未处理动物的泌乳乳腺中发现的MMTV蛋白量相比，致瘤性激素处理导致乳腺中MMTV蛋白量增加。乳腺肿瘤中MMTV蛋白的水平低于乳腺中的水平。
