Dasgupta U B, Summers W C
Mol Gen Genet. 1980;178(3):617-23. doi: 10.1007/BF00337869.
Recombination frequencies for two sets of genetic markers of herpes simplex virus were determined in various host cells with and without ultraviolet irradiation of the virus. UV irradiation increased the recombination frequency in all the cell types studied in direct proportion to the unrepaired lethal damage. In human skin fibroblasts derived from a patient with xeroderma pigmentosum (XP) of complementation group A, a given dose of UV stimulated recombination more than that in fibroblasts from normal individuals. On the other hand, UV stimulation of HSV recombination was slightly less than normal in fibroblasts derived from a patient with a variant form XP and from an ataxia telangiectasia patient. Caffeine, an agent known to inhibit repair of UV damage, reduced recombination in most of the cell types studied and did not suppress the UV-induced increase in recombination. These findings suggest that for virus DNA with the same number of unrepaired UV-lesions, each of the tested cell types promoted HSV-recombination to an equivalent extent.
在有或没有对病毒进行紫外线照射的情况下，在各种宿主细胞中测定了单纯疱疹病毒两组遗传标记的重组频率。紫外线照射使所有研究的细胞类型中的重组频率增加，且与未修复的致死损伤成正比。在来自互补组A的着色性干皮病（XP）患者的人皮肤成纤维细胞中，给定剂量的紫外线比正常个体的成纤维细胞更能刺激重组。另一方面，来自变异型XP患者和共济失调毛细血管扩张症患者的成纤维细胞中，紫外线对HSV重组的刺激略低于正常水平。咖啡因是一种已知可抑制紫外线损伤修复的物质，它降低了大多数研究细胞类型中的重组，并且没有抑制紫外线诱导的重组增加。这些发现表明，对于具有相同数量未修复紫外线损伤的病毒DNA，每种测试的细胞类型促进HSV重组的程度相当。
