Guschin I S, Uvnäs B
Acta Physiol Scand. 1976 Oct;98(2):168-74. doi: 10.1111/j.1748-1716.1976.tb00236.x.
Mast cells from the peritoneal and pleural cavities of actively sensitized rats were isolated and incubated with biogenic amines (5-hydroxytryptamine and dopamine) with or without pretreatment with specific antigen. An anaphylactic reaction resulting in the release of 20-25% of the histamine in the cells led to a slightly reduced amine uptake. At concentrations which induced histamine release comparable to that during the anaphylactic reaction compound 48/80 had a similar effect on the uptake of the two amines. Histamine release induced by higher concentrations of compound 48/80 led to a more pronounced reduction in the uptake of the amines, the reduction being roughly proportional to the extent of the histamine release. It is concluded that the reduction in the in vitro amine uptake after anaphylactic and compound 48/80-induced histamine release is due to the fact that there are a fewer intact granules capable of storing histamine and not primarily due to a damage to the mechanisms by which mast cells take up biogenic amines in vitro. The observations further strengthen the view that anaphylactic and compound 48/80-induced histamine release are non-cytolytic processes.
从主动致敏大鼠的腹腔和胸腔中分离出肥大细胞，将其与生物胺（5-羟色胺和多巴胺）一起孵育，其中一部分在孵育前先用特异性抗原进行预处理。导致细胞内20%-25%的组胺释放的过敏反应，使胺摄取略有减少。在诱导组胺释放的浓度与过敏反应期间相当的情况下，化合物48/80对两种胺的摄取有类似影响。更高浓度的化合物48/80诱导的组胺释放导致胺摄取更明显的减少，这种减少大致与组胺释放的程度成正比。得出的结论是，过敏反应和化合物48/80诱导组胺释放后，体外胺摄取减少是由于能够储存组胺的完整颗粒较少，而不是主要由于肥大细胞在体外摄取生物胺的机制受到损害。这些观察结果进一步强化了这样一种观点，即过敏反应和化合物48/80诱导的组胺释放是非细胞溶解过程。
