Bergendorff A
Acta Physiol Scand. 1975 Sep;95(1):133-41. doi: 10.1111/j.1748-1716.1975.tb10035.x.
Mast cells isolated from rat peritoneal and pleural cavities were incubated in vitro with radioactively-labelled histamine (Hi), 5-hydroxytryptamine (5-HT), dopamine (DA), noradrenaline (NA), tyramine (TA), phenylethylamine (PhEA), trptamine (TrpA), ephedrine (Eph) or amphetamine (Amph). All these amines were taken up by the mast cells. The dose-response curves for the compound 48/80-induced release of endogenous Hi and for the various amines taken up by the cells were compared. The release curves for Hi, 5-HT, DA, NA and TA were found to be similar to that for endogenous Hi, while those for PhEA, TrpA, Eph and Amph were different from that for endogenous Hi. The uptake of Hi, 5-HT, DA, PhEA, TrpA and Eph into granules in mast cells was studied. Membrane-bound granules were obtained by sonication of mast cells incubated with the respective amine, followed by differential contrifugation. The amine content of these granules was then measured. Hi, 5-HT and DA were found to be mainly localized to the granules, while a smaller proportion of the PhEA, TrpA and Eph was found there, the rest being located extragranularly. The present results suggest that, when taken up by rat mast cells, even amines which are not endogenous to the cells are stored in the same way as the endogenous amines Hi and 5-HT.
从大鼠腹腔和胸腔分离出的肥大细胞，在体外与放射性标记的组胺（Hi）、5-羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NA）、酪胺（TA）、苯乙胺（PhEA）、色胺（TrpA）、麻黄碱（Eph）或苯丙胺（Amph）一起孵育。所有这些胺都被肥大细胞摄取。比较了化合物48/80诱导的内源性Hi释放以及细胞摄取的各种胺的剂量反应曲线。发现Hi、5-HT、DA、NA和TA的释放曲线与内源性Hi的相似，而PhEA、TrpA、Eph和Amph的释放曲线与内源性Hi的不同。研究了Hi、5-HT、DA、PhEA、TrpA和Eph在肥大细胞颗粒中的摄取情况。通过将与相应胺孵育的肥大细胞超声处理，然后进行差速离心，获得膜结合颗粒。然后测量这些颗粒中的胺含量。发现Hi、5-HT和DA主要定位于颗粒中，而在颗粒中发现的PhEA、TrpA和Eph比例较小，其余位于颗粒外。目前的结果表明，当被大鼠肥大细胞摄取时，即使是细胞非内源性的胺，其储存方式也与内源性胺Hi和5-HT相同。
