Hynie S, Lanefelt F, Fredholm B B
Acta Pharmacol Toxicol (Copenh). 1980 Jul;47(1):58-65. doi: 10.1111/j.1600-0773.1980.tb02026.x.
The effects of ethanol, acetone and dimethylsulfoxide (DMSO) were tested on the accumulation of cyclic AMP in human peripheral blood lymphocytes in vitro. Isoproterenol (1.0X10(-8) - 1.0X10(-4)M), PGE2 (3X10(-8)-3X10(-6)M), adenosine (10(-7) - 10(-4)M) and phenylisopropyladenosine (10(-8) - 10(-4)M) caused a dose dependent increase in cyclic AMP accumulation. Over the entire range of concentration of stimulating drugs, ethanol caused an enhanced accumulation of cyclic AMP. At temperatures between 15 degrees and 30 degrees the effect of ethanol rose with increasing concentration from 0.2-6%. At 37 degrees and 40 degrees, 6% ethanol had less stimulatory effect than 2% ethanol. The effect of ethanol was shared by acetone and to a minor extent by DMSO, and was present also when phosphodiesterase was inhibited by isobutylmethylxanthine. It is suggested that ethanol enhances adenylate cyclase activity as a consequence of altered cell membrane fluidity. Since the effects on cyclic AMP accumulation can be observed already at rather low concentration of the solvents they may be of toxicological significance.
在体外对人外周血淋巴细胞中环状AMP的积累测试了乙醇、丙酮和二甲基亚砜（DMSO）的作用。异丙肾上腺素（1.0×10⁻⁸ - 1.0×10⁻⁴M）、前列腺素E2（3×10⁻⁸ - 3×10⁻⁶M）、腺苷（10⁻⁷ - 10⁻⁴M）和苯异丙基腺苷（10⁻⁸ - 10⁻⁴M）引起环状AMP积累呈剂量依赖性增加。在刺激药物的整个浓度范围内，乙醇导致环状AMP积累增强。在15摄氏度至30摄氏度之间的温度下，乙醇的作用随浓度从0.2% - 6%增加而增强。在37摄氏度和40摄氏度时，6%乙醇的刺激作用小于2%乙醇。丙酮也有乙醇的这种作用，二甲基亚砜在较小程度上也有，并且当磷酸二酯酶被异丁基甲基黄嘌呤抑制时这种作用也存在。提示乙醇由于改变细胞膜流动性而增强腺苷酸环化酶活性。由于在相当低的溶剂浓度下就能观察到对环状AMP积累的影响，它们可能具有毒理学意义。
