Solanki V, Murray A W
J Invest Dermatol. 1982 Apr;78(4):264-6. doi: 10.1111/1523-1747.ep12506839.
The effect of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) on cyclic adenosine 3',5'-monophosphate (cyclic AMP) level in adult mouse skin in response to ischemia was examined. The incubation of skin pieces in a buffered salts medium at 37 degrees C resulted in a rapid accumulation of cyclic AMP. In mouse skin pieces maximum accumulation (about 6 times the basal level) occurred after 2 min incubation and was followed by a rapid decline in the cyclic AMP level. This "ischemic" rise in epidermal cyclic AMP was greatly reduced if skin was used 16 hr after a single application of 17 nmoles of TPA. The effect of TPA on cyclic AMP accumulation in response to ischemia was first observed at 1 hr after TPA treatment and was maximal at 4 hr. The lack of "ischemic" response in TPA-treated skin was not related to an increase in the activity of cyclic AMP phosphodiesterase after TPA application. In addition, the accumulation of cyclic AMP in skin in response to both ischemia and exposure to isoproterenol, adenosine, histamine, or prostaglandin E2 (PGE2) was not observed in skin treated with the tumor promoter TPA.
研究了12-0-十四烷酰佛波醇-13-乙酸酯（TPA）对成年小鼠皮肤在缺血状态下环磷腺苷（环AMP）水平的影响。将皮肤切片在37℃的缓冲盐培养基中孵育会导致环AMP迅速积累。在小鼠皮肤切片中，孵育2分钟后出现最大积累量（约为基础水平的6倍），随后环AMP水平迅速下降。如果在单次应用17纳摩尔TPA后16小时使用皮肤，则表皮中环AMP的这种“缺血性”升高会大大降低。TPA处理后1小时首次观察到TPA对缺血诱导的环AMP积累的影响，4小时时达到最大。TPA处理的皮肤中缺乏“缺血性”反应与TPA应用后环AMP磷酸二酯酶活性增加无关。此外，在用肿瘤促进剂TPA处理的皮肤中，未观察到皮肤对缺血以及异丙肾上腺素、腺苷、组胺或前列腺素E2（PGE2）暴露的环AMP积累情况。
