一种来自大鼠肝脏的微粒体内切核糖核酸酶。
A microsomal endoribonuclease from rat liver.
作者信息
Kumagai H, Igarashi K, Takayama T, Watanabe K, Sugimoto K, Hirose S
出版信息
Biochim Biophys Acta. 1980 Jul 29;608(2):324-31. doi: 10.1016/0005-2787(80)90178-1.
An endoribonuclease has been purified about 320-fold from the microsomes of rat liver. The enzyme had an apparent molecular weight of 54 000-58 000 and produced oligonucleotides, each consisting of 3-7 nucleotides from poly(A) and poly(U). No mononucleotide was obtained by the enzymatic hydrolysis of poly(A) and poly(U) under standard coditions. The relative rates of breakdown of synthetic polynucleotides by the enzyme under standard conditions were in the order poly(U) = poly(A) > poly(C). Divalent cations (Mg2+ or Mn2+) was required for the enzymatic activity, but monovalent cations (Na+, K+ or NH4+) inhibited the enzyme. The breakdown of poly(C) and poly(U) by the enzyme was inhibited by spermine, but that of poly(A) was not influenced by spermine. The enzyme was inhibited by p-chloromercuribenzoate and poly(G), but not by rat-liver ribonuclease-inhibitor and anti-RNase A serum.
一种核糖核酸内切酶已从大鼠肝脏微粒体中纯化出来，纯化倍数约为320倍。该酶的表观分子量为54000 - 58000，能产生寡核苷酸，每个寡核苷酸由来自聚腺苷酸（poly(A)）和聚尿苷酸（poly(U)）的3 - 7个核苷酸组成。在标准条件下，通过该酶对聚腺苷酸和聚尿苷酸进行酶促水解未得到单核苷酸。在标准条件下，该酶对合成多核苷酸的相对降解速率顺序为：聚尿苷酸 = 聚腺苷酸 > 聚胞苷酸（poly(C)）。酶活性需要二价阳离子（Mg2+或Mn2+），但一价阳离子（Na+、K+或NH4+）会抑制该酶。精胺会抑制该酶对聚胞苷酸和聚尿苷酸的降解，但对聚腺苷酸的降解没有影响。该酶受到对氯汞苯甲酸和聚鸟苷酸（poly(G)）的抑制，但不受大鼠肝脏核糖核酸酶抑制剂和抗核糖核酸酶A血清的抑制。
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