环磷酸腺苷、环磷酸鸟苷和钙调蛋白在盘基网柄菌中的免疫组织化学定位。

Immunohistochemical localization of cyclic AMP, cyclic GMP and calmodulin in Dictyostelium discoideum.

作者信息

Mato J M, Steiner A L

出版信息

Cell Biol Int Rep. 1980 Jul;4(7):641-8. doi: 10.1016/0309-1651(80)90202-7.

Abstract

Using an indirect immunofluorescence technique cyclic AMP, cyclic GMP and calmodulin have been localized in Dictyostelium discoideum cells. The bound cyclic AMP is localized throughout the cell. Cyclic GMP and calmodulin are markedly localized in the nuclear area of the cell. No changes in the pattern of staining was observed during differentiation. Chemotactic stimulation induced a transient increase of cyclic GMP staining.

摘要

运用间接免疫荧光技术，已在盘基网柄菌细胞中定位了环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）和钙调蛋白。结合的cAMP在整个细胞中均有定位。cGMP和钙调蛋白显著定位于细胞的核区域。在分化过程中未观察到染色模式的变化。趋化刺激诱导cGMP染色短暂增加。