Flandroy L, Cheung W Y, Steiner A L
Cell Tissue Res. 1983;233(3):639-46. doi: 10.1007/BF00212231.
Cyclic guanosine 3',5' monophosphate (cGMP), cGMP-dependent protein kinase, calmodulin and cyclic adenosine 3',5' monophosphate (cAMP) were localized in the uterus of the immature rat by an indirect immunofluorescence technique. cGMP, cGMP-dependent protein kinase and calmodulin were detected predominantly along epithelial and myometrial plasma membranes and in the adjacent cytoplasm. In contrast, cAMP immunoreactive material was found principally in the cytoplasm of connective tissue. After administration of 17 beta-estradiol, similar time-dependent changes were observed in the localization of cGMP, cGMP-dependent protein kinase and calmodulin in all uterine cell types. For the three compounds, nucleolar-like distribution of the immunofluorescence appeared approximately 12 h after treatment. A more dispersed, reticular distribution of the nuclear fluorescent staining was observed 20-24 h after hormonal treatment. Estrogen did not affect the localization of cAMP. The simultaneous mobilization of cGMP, cGMP-dependent protein kinase and calmodulin towards the same nuclear loci suggests concerted roles for these three molecules in nuclear metabolic processes during the development of the uterotrophic action of estrogens.
通过间接免疫荧光技术，在未成熟大鼠子宫中定位了环鸟苷3',5'-单磷酸（cGMP）、cGMP依赖性蛋白激酶、钙调蛋白和环腺苷3',5'-单磷酸（cAMP）。cGMP、cGMP依赖性蛋白激酶和钙调蛋白主要在子宫内膜和肌层质膜以及相邻的细胞质中检测到。相比之下，cAMP免疫反应性物质主要存在于结缔组织的细胞质中。给予17β-雌二醇后，在所有子宫细胞类型中，cGMP、cGMP依赖性蛋白激酶和钙调蛋白的定位观察到类似的时间依赖性变化。对于这三种化合物，免疫荧光的核仁样分布在处理后约12小时出现。激素处理后20-24小时，观察到核荧光染色呈更分散的网状分布。雌激素不影响cAMP的定位。cGMP、cGMP依赖性蛋白激酶和钙调蛋白同时向相同的核位点移动，表明这三种分子在雌激素子宫营养作用发展过程中的核代谢过程中发挥协同作用。
