Skala J P, Hahn P, Knight B L
Biochim Biophys Acta. 1980 Sep 1;631(3):420-7. doi: 10.1016/0304-4165(80)90018-5.
The concentrations of cyclic AMP and cyclic GMP in brown fat and liver of both suckling and adult rats at fixed times after injection of insulin (2.5 U/100 g body weight) or prednisolone (2.5 mg/100 g body weight) were compared with the activity of phosphoenolpyruvate carboxykinase assayed 24 h after the injections. A stimulus that produced an increase in cyclic AMP content also produced an increase in the enzyme activity. If the content of cyclic GMP was also increased there was no rise in phosphoenolpyruvate carboxykinase activity. A rise in the content of cyclic GMP alone was associated with a reduction in the activity of the enzyme. These preliminary results indicate that cyclic AMP could be involved in the induction of phosphoenolpyruvate carboxykinase and that cyclic GMP may somehow be related to its repression. The known differences in the response of phosphoenolpyruvate carboxykinase activity to insulin and prednisolone in different tissues and at different stages of ontogenic development may thus be linked to differences in the responsiveness of enzymes concerned with the metabolism of cyclic nucleotides.
在注射胰岛素（2.5 U/100 g体重）或泼尼松龙（2.5 mg/100 g体重）后的固定时间，比较了哺乳大鼠和成年大鼠棕色脂肪及肝脏中环状AMP和环状GMP的浓度，并与注射后24小时测定的磷酸烯醇丙酮酸羧激酶活性进行了比较。能使环状AMP含量增加的刺激也会使该酶活性增加。如果环状GMP含量也增加，则磷酸烯醇丙酮酸羧激酶活性不会升高。仅环状GMP含量的升高与该酶活性的降低有关。这些初步结果表明，环状AMP可能参与了磷酸烯醇丙酮酸羧激酶的诱导，而环状GMP可能以某种方式与其抑制作用有关。因此，在不同组织以及个体发育的不同阶段，磷酸烯醇丙酮酸羧激酶活性对胰岛素和泼尼松龙反应的已知差异，可能与参与环状核苷酸代谢的酶的反应性差异有关。
