被动免疫疗法可阻止内源性莫洛尼病毒在BALB/Mo小鼠中的表达以及前病毒DNA的扩增。
Passive immunotherapy prevents expression of endogenous Moloney virus and amplification of proviral DNA in BALB/Mo mice.
作者信息
Nobis P, Jaenisch R
出版信息
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3677-81. doi: 10.1073/pnas.77.6.3677.
Abstract
BALB/Mo mice carrying the Moloney murine leukemia virus (M-MuLV) as an endogenous virus become viremic soon after birth and develop leukemia at a later age. M-MuLV-specific gene expression and an increase of virus-specific DNA copies in lymphatic target organs are characteristics of the preleukemic phase. Passive immunotherapy of new born BALB/Mo mice with anti-gp70 glycoprotein or anti-M-MuLV serum prevented viremia and delayed significantly the subsequent development of leukemia. Molecular hybridization experiments showed that both virus-specific genome transcription and virus-specific DNA amplification could be completely suppressed by antiserum treatment. Thus virus-specific RNA concentrations in target organs of immunized BALB/Mo mice of 6 months or older were as low as in normal BALB/c mice. This is an age at which untreated BALB/Mo mice have already developed malignant lymphoma. Our experiments demonstrate that treatment with antiserum interferes with the early events of virus expression and thus prevents the subsequent steps leading to leukemia.
摘要
携带莫洛尼鼠白血病病毒（M-MuLV）作为内源性病毒的BALB/Mo小鼠在出生后不久就会出现病毒血症，并在后期发展为白血病。M-MuLV特异性基因表达以及淋巴靶器官中病毒特异性DNA拷贝数的增加是白血病前期的特征。用抗gp70糖蛋白或抗M-MuLV血清对新生BALB/Mo小鼠进行被动免疫治疗可预防病毒血症，并显著延迟随后白血病的发展。分子杂交实验表明，抗血清治疗可完全抑制病毒特异性基因组转录和病毒特异性DNA扩增。因此，6个月及以上免疫的BALB/Mo小鼠靶器官中的病毒特异性RNA浓度与正常BALB/c小鼠一样低。在这个年龄，未经治疗的BALB/Mo小鼠已经发展为恶性淋巴瘤。我们的实验表明，抗血清治疗会干扰病毒表达的早期事件，从而阻止导致白血病的后续步骤。
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本文引用的文献
1
Properties of mouse leukemia viruses. XIV. Prevention of spontaneous AKR leukemia by treatment with group-specific antibody against the major virus gp71 glycoprotein.小鼠白血病病毒的特性。十四、用针对主要病毒糖蛋白gp71的群特异性抗体治疗预防自发性AKR白血病
Virology. 1977 Nov;83(1):207-10. doi: 10.1016/0042-6822(77)90224-0.
2
"Spontaneous" leukemia developing in C3H mice following inoculation in infancy, with AK-leukemic extracts, or AK-embrvos.在婴儿期接种AK白血病提取物或AK胚胎后，C3H小鼠中发生的“自发性”白血病。
Proc Soc Exp Biol Med. 1951 Jan;76(1):27-32.
3
A simple chromatographic method for preparation of gamma globulin.一种制备γ球蛋白的简易色谱法。
Proc Soc Exp Biol Med. 1960 Jan;103:250-2. doi: 10.3181/00379727-103-25476.
4
Conformation of free and of integrated Moloney leukemia virus proviral DNA in preleukemic and leukemic BALB/Mo mice.前白血病和白血病BALB/Mo小鼠中游离及整合的莫洛尼白血病病毒前病毒DNA的构象
Virology. 1980 Feb;101(1):111-23. doi: 10.1016/0042-6822(80)90488-2.
5
Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains.内源性鼠白血病病毒基因表达的宿主控制：高白血病和低白血病小鼠品系中病毒蛋白的浓度
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3682-6. doi: 10.1073/pnas.71.9.3682.
6
Host-gene control of type-C RNA tumor virus expression and tumorigenesis in inbred mice.近交系小鼠中C型RNA肿瘤病毒表达和肿瘤发生的宿主基因控制
Proc Natl Acad Sci U S A. 1973 May;70(5):1450-5. doi: 10.1073/pnas.70.5.1450.
7
Quantitative studies of naturally occurring murine leukemia virus infection of AKR mice.对AKR小鼠自然发生的鼠白血病病毒感染的定量研究。
J Exp Med. 1972 Feb 1;135(2):429-36. doi: 10.1084/jem.135.2.429.
8
Plaque assay techniques for murine leukemia viruses.小鼠白血病病毒的噬斑测定技术
Virology. 1970 Dec;42(4):1136-9. doi: 10.1016/0042-6822(70)90362-4.
9
10
Germ line integration and Mendelian transmission of the exogenous Moloney leukemia virus.外源性莫洛尼白血病病毒的种系整合及孟德尔式传递。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1260-4. doi: 10.1073/pnas.73.4.1260.
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