Dwek R D, Kobrin L H, Grossman N, Ron E Z
J Bacteriol. 1980 Oct;144(1):17-21. doi: 10.1128/jb.144.1.17-21.1980.
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
我们描述了一种通过使用Percoll密度梯度来获得细菌(大肠杆菌B和K-12以及枯草芽孢杆菌168)和裂殖酵母(粟酒裂殖酵母)同步分裂细胞的方法。