通过Percoll梯度实现微生物细胞分裂的同步化。
Synchronization of cell division in microorganisms by percoll gradients.
作者信息
Dwek R D, Kobrin L H, Grossman N, Ron E Z
出版信息
J Bacteriol. 1980 Oct;144(1):17-21. doi: 10.1128/jb.144.1.17-21.1980.
Abstract
We describe a method for obtaining synchronously dividing cells of bacteria (Escherichia coli B and K-12 and Bacillus subtilis 168) and fission yeasts (Schizosaccharomyces pombe) by the use of Percoll density gradients.
摘要
我们描述了一种通过使用Percoll密度梯度来获得细菌（大肠杆菌B和K-12以及枯草芽孢杆菌168）和裂殖酵母（粟酒裂殖酵母）同步分裂细胞的方法。
相似文献
1
Synchronization of cell division in microorganisms by percoll gradients.通过Percoll梯度实现微生物细胞分裂的同步化。
J Bacteriol. 1980 Oct;144(1):17-21. doi: 10.1128/jb.144.1.17-21.1980.
2
Variation in Escherichia coli buoyant density measured in Percoll gradients.在Percoll梯度中测得的大肠杆菌浮力密度的变化。
J Bacteriol. 1981 Oct;148(1):58-63. doi: 10.1128/jb.148.1.58-63.1981.
3
Entamoeba invadens and E. histolytica: separation and purification of precysts and cysts by centrifugation on discontinuous density gradients of Percoll.侵袭内阿米巴和溶组织内阿米巴：通过在不连续的Percoll密度梯度上离心分离和纯化前包囊和包囊。
Exp Parasitol. 1983 Jun;55(3):265-9. doi: 10.1016/0014-4894(83)90022-x.
4
Cell Cycle Synchronization of Schizosaccharomyces pombe by Lactose Gradient Centrifugation to Isolate Small Cells.通过乳糖梯度离心法同步粟酒裂殖酵母细胞周期以分离小细胞
Cold Spring Harb Protoc. 2016 Jun 1;2016(6):2016/6/pdb.prot091249. doi: 10.1101/pdb.prot091249.
5
Density gradients prepared from colloidal silical particles coated by polyvinylpyrrolidone (Percoll). II. Radioactive labeling of Percoll.由聚乙烯吡咯烷酮包被的胶体硅颗粒制备的密度梯度（Percoll）。II. Percoll的放射性标记。
Anal Biochem. 1978 Jul 15;88(1):283-4. doi: 10.1016/0003-2697(78)90420-7.
6
Production of Ficoll, Percoll, and albumin gradients by the freeze-thaw method.通过冻融法制备菲可、派可和白蛋白梯度液。
Prep Biochem. 1979;9(2):149-56. doi: 10.1080/00327487908061680.
7
Analysis of the Schizosaccharomyces pombe Cell Cycle.粟酒裂殖酵母细胞周期分析
Cold Spring Harb Protoc. 2016 Sep 1;2016(9):2016/9/pdb.top082800. doi: 10.1101/pdb.top082800.
8
Comparative study of eosinophil purification on Nycodenz, Metrizamide and Percoll density gradients.Nycodenz、Metrizamide和Percoll密度梯度上嗜酸性粒细胞纯化的比较研究。
Eur J Haematol. 1987 Aug;39(2):148-53. doi: 10.1111/j.1600-0609.1987.tb00745.x.
9
Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).由聚乙烯吡咯烷酮包被的胶体二氧化硅颗粒（Percoll）制备的密度梯度。
Anal Biochem. 1978 Jul 15;88(1):271-82. doi: 10.1016/0003-2697(78)90419-0.
10
The viability of cells grown or centrifuged in a new density gradient medium, Percoll(TM).在新型密度梯度介质Percoll™中生长或离心的细胞的活力。
Exp Cell Res. 1977 Dec;110(2):449-57. doi: 10.1016/0014-4827(77)90311-1.
引用本文的文献
1
A Miniaturized Percoll Gradient Method for Isolation of Quiescent Cells of Yeast.一种用于分离酵母静止细胞的小型化 Percoll 梯度法。
Bio Protoc. 2025 Apr 20;15(8):e5279. doi: 10.21769/BioProtoc.5279.
2
Coordinated Regulation of Rsd and RMF for Simultaneous Hibernation of Transcription Apparatus and Translation Machinery in Stationary-Phase .Rsd和RMF的协同调控实现静止期转录装置和翻译机器的同步休眠
Front Genet. 2019 Dec 4;10:1153. doi: 10.3389/fgene.2019.01153. eCollection 2019.
3
Flagellar Synchronization Is a Simple Alternative to Cell Cycle Synchronization for Ciliary and Flagellar Studies.鞭毛同步化是用于纤毛和鞭毛研究的细胞周期同步化的一种简单替代方法。
mSphere. 2017 Mar 8;2(2). doi: 10.1128/mSphere.00003-17. eCollection 2017 Mar-Apr.
4
Use of the sucrose gradient method for bacterial cell cycle synchronization.使用蔗糖梯度法进行细菌细胞周期同步化。
J Microbiol Biol Educ. 2012 May 3;13(1):50-3. doi: 10.1128/jmbe.v13i1.346. Print 2012.
5
Interplay between intrinsic noise and the stochasticity of the cell cycle in bacterial colonies.细菌菌落中内在噪声与细胞周期随机性的相互作用。
Biophys J. 2010 Jun 2;98(11):2459-68. doi: 10.1016/j.bpj.2010.02.045.
6
Density-dependent sorting of physiologically different cells of Vibrio parahaemolyticus.副溶血性弧菌生理上不同细胞的密度依赖性分选
Appl Environ Microbiol. 2003 Jun;69(6):3569-72. doi: 10.1128/AEM.69.6.3569-3572.2003.
7
Changes in cell dimensions during amino acid starvation of Escherichia coli.大肠杆菌氨基酸饥饿期间细胞尺寸的变化
J Bacteriol. 1982 Oct;152(1):35-41. doi: 10.1128/jb.152.1.35-41.1982.
8
Monitoring microbial numbers in food by density centrifugation.通过密度离心法监测食品中的微生物数量。
Appl Environ Microbiol. 1983 Mar;45(3):1156-9. doi: 10.1128/aem.45.3.1156-1159.1983.
9
Isopycnic separation of Escherichia coli cultures possessing colonization factor antigen I.具有定居因子抗原I的大肠杆菌培养物的等密度分离
J Clin Microbiol. 1982 Jun;15(6):1074-6. doi: 10.1128/jcm.15.6.1074-1076.1982.
10
Variation in Escherichia coli buoyant density measured in Percoll gradients.在Percoll梯度中测得的大肠杆菌浮力密度的变化。
J Bacteriol. 1981 Oct;148(1):58-63. doi: 10.1128/jb.148.1.58-63.1981.
本文引用的文献
1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
COMPARISON OF SYNCHRONOUS AND SYNCHRONIZED CELL DIVISION.同步和同步化细胞分裂的比较
Exp Cell Res. 1964 Jan;33:89-98. doi: 10.1016/s0014-4827(64)81016-8.
3
Physical methods for obtaining synchronous culture of Escherichia coli.获得大肠杆菌同步培养物的物理方法。
J Bacteriol. 1956 May;71(5):542-6. doi: 10.1128/jb.71.5.542-546.1956.
4
The induction of cellular and nuclear division in Salmonella typhimurium by means of temperature shifts.通过温度变化诱导鼠伤寒沙门氏菌的细胞分裂和核分裂。
Biochim Biophys Acta. 1954 Nov;15(3):345-56. doi: 10.1016/0006-3002(54)90036-0.
5
Synchronous growth of enteric bacteria.肠道细菌的同步生长。
J Bacteriol. 1970 Sep;103(3):789-92. doi: 10.1128/jb.103.3.789-792.1970.
6
Deoxyribonucleic acid synthesis during the division cycle of Escherichia coli: a comparison of strains B-r, K-12, 15, and 15T- under conditions of slow growth.大肠杆菌分裂周期中的脱氧核糖核酸合成：B-r、K-12、15和15T-菌株在生长缓慢条件下的比较
J Bacteriol. 1974 Mar;117(3):1216-23. doi: 10.1128/jb.117.3.1216-1223.1974.
7
Synchronization of cell division in Escherichia coli by amino acid starvation: strain specificity.通过氨基酸饥饿使大肠杆菌中的细胞分裂同步：菌株特异性。
J Bacteriol. 1975 Jul;123(1):374-6. doi: 10.1128/jb.123.1.374-376.1975.
8
Quantitative assessment of the synchronization of cell populations.细胞群体同步性的定量评估。
J Gen Microbiol. 1979 Jan;110(1):225-7. doi: 10.1099/00221287-110-1-225.
9
Morphological analysis of the division cycle of two Escherichia coli substrains during slow growth.缓慢生长期间两种大肠杆菌菌株分裂周期的形态学分析
J Bacteriol. 1977 Jul;131(1):270-9. doi: 10.1128/jb.131.1.270-279.1977.
10
Control of cell division in Escherichia coli: effect of amino acid starvation.大肠杆菌中细胞分裂的调控：氨基酸饥饿的影响
J Bacteriol. 1977 Feb;129(2):569-73. doi: 10.1128/jb.129.2.569-573.1977.
Zotero 插件