Hamlet M A, Rorie D K, Tyce G M
Am J Physiol. 1980 Oct;239(4):H450-H456. doi: 10.1152/ajpheart.1980.239.4.H450.
Studies were performed to determine whether estradiol (E2) has a direct action on the release and disposition of norepinephrine (NE) from adrenergic nerve endings in isolated superfused canine saphenous veins. [3H]NE and is labeled metabolites were separated by column chromatography with measurement by liquid scintillation spectrometry. An increase in the spontaneous overflow of total 3H, [3H]NE, and [3H]dihydroxyphenylglycol ([3H]DOPEG) that occurred with 1 and 10 microgram/ml E2 in the superfusing medium suggested that E2 either induced NE release or interfered with intraneuronal NE storage. During electrical stimulation (ES), release of [3H]NE and [3H]DOPEG exceeded controls with 1 and 10 microgram/ml E2 in the superfusate, yet total 3H was little changed. The evoked release of [3H]DOPEG showed less of an elevation over its spontaneous efflux in E2-treated veins than in nontreated veins, suggesting that E2 may block neuronal reuptake of released NE. The efflux of O-methylated deaminated metabolites during and after ES was decreased by E2 treatment, suggesting also an inhibition of extraneuronal uptake of NE.
开展了多项研究，以确定雌二醇（E2）是否对离体灌注的犬隐静脉中肾上腺素能神经末梢去甲肾上腺素（NE）的释放和处置具有直接作用。[3H]NE及其标记代谢物通过柱色谱法分离，并通过液体闪烁光谱法进行测量。在灌注培养基中加入1和10微克/毫升E2时，总3H、[3H]NE和[3H]二羟基苯乙二醇（[3H]DOPEG）的自发溢出增加，这表明E2要么诱导NE释放，要么干扰神经元内NE的储存。在电刺激（ES）期间，灌注液中加入1和10微克/毫升E2时，[3H]NE和[3H]DOPEG的释放超过对照组，但总3H变化不大。与未处理的静脉相比，E2处理的静脉中，[3H]DOPEG的诱发释放相对于其自发流出的升高幅度较小，这表明E2可能会阻断释放的NE的神经元再摄取。E2处理可降低ES期间及之后O-甲基化脱氨基代谢物的流出，这也表明对NE的神经元外摄取有抑制作用。
