Lunn J J, Rorie D K
Anesthesiology. 1984 Oct;61(4):377-84. doi: 10.1097/00000542-198410000-00004.
The effect of halothane on the release and metabolism of norepinephrine during resting conditions and in response to electrical stimulation at 2 Hz was studied in isolated superfused segments od dog saphenous vein. Liquid chromatography with electrochemical detection was used to measure endogenous norepinephrine overflowing in response to electrical stimulation and the content of norepinephrine remaining in the tissue after stimulation. In other preparations, norepinephrine stores were labeled with [3H]norepinephrine, and measurements were made of [3H]norepinephrine and its metabolites (separated by column chromatography) in superfusates. Radiolabeled metabolites of norepinephrine produced intraneuronally (3,4-dihydroxyphenylglycol) and extraneuronally (O-methylated) were quantitated by liquid scintillation spectrometry. Electrically stimulated release and overflow of endogenous norepinephrine was decreased 11.9% at 0.75% halothane, 17.7% at 1.5% and 19.2% at 2.5% halothane. At each halothane concentration studied, the per cent of tissue NE content released in response to electrical stimulation was less. Halothane decreased the fraction of radioactivity lost during basal conditions and during stimulation. Less oxidative deamination of norepinephrine occurred in the presence of halothane.
在离体灌流的犬隐静脉段中，研究了氟烷在静息状态下以及对2Hz电刺激反应时对去甲肾上腺素释放和代谢的影响。采用电化学检测的液相色谱法来测量电刺激引起的内源性去甲肾上腺素溢出以及刺激后组织中剩余的去甲肾上腺素含量。在其他制剂中，用[3H]去甲肾上腺素标记去甲肾上腺素储存库，并测量灌流液中[3H]去甲肾上腺素及其代谢产物（通过柱色谱分离）。通过液体闪烁光谱法对神经元内产生的（3,4-二羟基苯乙二醇）和神经元外产生的（O-甲基化）去甲肾上腺素放射性标记代谢产物进行定量。在0.75%氟烷时，电刺激引起的内源性去甲肾上腺素释放和溢出减少11.9%，在1.5%时减少17.7%，在2.5%时减少19.2%。在所研究的每个氟烷浓度下，电刺激引起的组织NE含量释放百分比均较低。氟烷降低了基础状态和刺激过程中放射性损失的比例。在氟烷存在的情况下，去甲肾上腺素的氧化脱氨基作用减少。
