[钙离子对兔空肠黏膜腺苷酸环化酶的抑制作用]
[Ca2+ inhibition of adenyl cyclase of rabbit jejunal mucosa].
作者信息
Iurkiv V A, Melikhov V I
出版信息
Biull Eksp Biol Med. 1980 Oct;90(10):430-2.
Ca2+ ions at a concentration of 10-7--10-3 M inhibit adenylate cyclase of the rabbit jejunum mucosa (K0.5= =10-4 M). This effect is not modified after the membrane extraction by 10-3 M EDTA solution with a high or low ionic strength or after removal from the membrane preparation of Ca2+-dependent thermostable regulatory protein. The inhibition by Ca2+ions was discovered also in the presence of adenylate cyclase irreversible activators (guanylylimidodiphosphate and NaF), which points to the spontaneous interaction of Ca2+ ions with the catalytic subunit of the adenylate cyclase complex.
浓度为10⁻⁷ - 10⁻³ M的Ca²⁺离子可抑制兔空肠黏膜的腺苷酸环化酶（K₀.₅ = 10⁻⁴ M）。在用高离子强度或低离子强度的10⁻³ M EDTA溶液进行膜提取后，或从膜制剂中去除Ca²⁺依赖性热稳定调节蛋白后，这种效应没有改变。在存在腺苷酸环化酶不可逆激活剂（鸟苷酰亚胺二磷酸和NaF）的情况下也发现了Ca²⁺离子的抑制作用，这表明Ca²⁺离子与腺苷酸环化酶复合物的催化亚基发生了自发相互作用。
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