Iurkiv V A, Melikhov V I
Biull Eksp Biol Med. 1981 Oct;91(10):432-4.
Activation of adenylate cyclase in membrane preparations of the rabbit small intestine mucosa by cholera enterotoxin in the presence of sodium 2,3-dithiopropanesulfate (DTPS) is similar to that in the presence of dithiothreitol (DTT). Both DTT and DTPS do not influence the activity of adenylate cyclase without cholera enterotoxin. DTPS activates cyclic 3,5-AMP phosphodiesterase of mucosa homogenates (K0.5 = 10(-5) M). Combined administration of cholera enterotoxin and DTPS in situ into isolated loops of the rabbit small intestine decreases the activating effect of cholera enterotoxin on adenylate cyclase and diminishes the content of cyclic AMP in the mucosa. The destruction of disulfide bonds of cholera enterotoxin by DTPS is assumed to lower its ability to penetrate the mucosal cells of the small intestine.
在2,3 - 二硫代丙烷磺酸钠（DTPS）存在的情况下，霍乱肠毒素对兔小肠黏膜膜制剂中腺苷酸环化酶的激活作用与在二硫苏糖醇（DTT）存在时相似。在没有霍乱肠毒素的情况下，DTT和DTPS均不影响腺苷酸环化酶的活性。DTPS激活黏膜匀浆的环3,5 - 腺苷酸磷酸二酯酶（K0.5 = 10(-5) M）。将霍乱肠毒素和DTPS原位联合注入兔小肠的分离肠袢中，会降低霍乱肠毒素对腺苷酸环化酶的激活作用，并减少黏膜中环磷酸腺苷的含量。推测DTPS对霍乱肠毒素二硫键的破坏会降低其穿透小肠黏膜细胞的能力。
