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[脱氧核糖核苷酸对大肠杆菌DNA聚合酶I 3'至5'核酸外切酶活性的抑制作用]

[Inhibition of the 3' to 5' exonuclease activity of the DNA polymerase I of Escherichia coli by deoxyribonucleotides].

作者信息

Granger M, Toulmé F, Hélène C

出版信息

C R Seances Acad Sci D. 1980 Sep 15;291(2):203-6.

PMID:6253102
Abstract

The 3' à 5' exonuclease activity of E. coli DNA-polymerase I is inhibited by nucleotides and deoxynucleotides at concentrations (< 1 mM) where polymerase activity is not affected. This inhibitory effect depends on the nature of the excised deoxynucleotide, excision of purines being much less inhibited than that of pyrimidines. It does not depend on the purine or pyrimidine nature of the inhibitor.

摘要

大肠杆菌DNA聚合酶I的3'→5'核酸外切酶活性在核苷酸和脱氧核苷酸浓度(<1 mM)下会受到抑制,而此时聚合酶活性不受影响。这种抑制作用取决于切除的脱氧核苷酸的性质,嘌呤的切除比嘧啶的切除受抑制程度小得多。它不取决于抑制剂的嘌呤或嘧啶性质。

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