Cooper G M, Copeland N G, Zelenetz A D, Krontiris T
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:1169-76. doi: 10.1101/sqb.1980.044.01.126.
NIH-3T3 cells were found to be transformable by RSV DNA in the absence of progeny virus production. Cells transformed by intact RSV DNAs contained rescuable RSV genomes that were integrated into cellular DNA and were colinear with unintegrated RSV DNA. NIH-3T3 cells were also transformable by subgenomic fragments of RSV DNA, synthesized in vitro or generated by restriction endonuclease digestion of intracellular RSV DNA. These cells did not contain rescuable RSV genomes but did contain RSV DNA fragments that efficiently induced transformation of NIH-3T3 cells in secondary transfection assays. Further analysis of the RSV DNA sequences present in these cells and transformation assays of defined fragments of RSV DNA may contribute to the elucidation of the sequences required for expression of the src gene of RSV. DNAs of the avian acute leukemia viruses MC29 and AEV also induced transformation of NIH-3T3 cells. The use of these cells as recipients may thus provide a system suitable for functional analysis of the transforming genes of avian leukemia viruses as well as sarcoma viruses.
在不产生子代病毒的情况下，发现NIH - 3T3细胞可被劳氏肉瘤病毒（RSV）DNA转化。由完整RSV DNA转化的细胞含有可拯救的RSV基因组，这些基因组整合到细胞DNA中，并且与未整合的RSV DNA共线。NIH - 3T3细胞也可被RSV DNA的亚基因组片段转化，这些片段是在体外合成的，或者是通过细胞内RSV DNA的限制性内切酶消化产生的。这些细胞不含有可拯救的RSV基因组，但确实含有RSV DNA片段，这些片段在二次转染试验中能有效地诱导NIH - 3T3细胞转化。对这些细胞中存在的RSV DNA序列进行进一步分析以及对RSV DNA的特定片段进行转化试验，可能有助于阐明RSV src基因表达所需的序列。禽急性白血病病毒MC29和AEV的DNA也能诱导NIH - 3T3细胞转化。因此，使用这些细胞作为受体可能会提供一个适合对禽白血病病毒以及肉瘤病毒的转化基因进行功能分析的系统。
