Carloni G, Stedman N, Hillova J, Marx M, Hill M
Intervirology. 1980;13(4):241-52. doi: 10.1159/000149131.
Chicken embryo fibroblasts were chronically infected by both RAV-1 and SR-E, and established SR-E-transformed quail cells were superinfected with RAV-1. About 75 and 50% of double-infected and superinfected cells, respectively, produced both parental viruses. The DNA purified from these cells contained infectious provirus of both parents and no detectable recombinant provirus. In assays using agar overlay, about 5% of the transformed foci that were induced by the DNA of double-infected, but not superinfected, cells were found to contain the progeny of both parents. This observation suggests that in double infections with avian retroviruses, DNA of both parent viruses may integrate into the host genome either in tandem or in close proximity.
鸡胚成纤维细胞被RAV - 1和SR - E长期感染，并且已建立的SR - E转化的鹌鹑细胞被RAV - 1超感染。分别约75%的双重感染细胞和50%的超感染细胞产生了两种亲代病毒。从这些细胞中纯化的DNA含有两种亲代的感染性前病毒，且未检测到重组前病毒。在使用琼脂覆盖的实验中，发现由双重感染而非超感染细胞的DNA诱导的转化灶中约5%含有两种亲代的子代。这一观察结果表明，在禽逆转录病毒的双重感染中，两种亲代病毒的DNA可能以串联或紧密相邻的方式整合到宿主基因组中。
