Miki T
Microbiol Immunol. 1980;24(5):429-38. doi: 10.1111/j.1348-0421.1980.tb02847.x.
The dependence on concanavalin A (Con A) concentration of agglutinability of some enveloped RNA viruses grown in transformed cells was compared with that of those grown in nontransformed cells. The avian oncoviruses were purified by centrifuging to equilibrium in a combination equilibrium: viscosity gradient of potassium tartrate and glycerol after conventional isopycnic sucrose density gradient centrifugation. Avian oncoviruses were more agglutinable with Con A when grown in transformed cells than when grown in nontransformed cells. Vesicular stomatitis virus grown in transformed cells was also more agglutinable than the virus grown in nontransformed cells. These results agree with the concept that the envelopes are modified by host cell transformation and that, therefore, viruses grown in transformed cells are expected to be more agglutinable with Con A than those grown in nontransformed cell.
将在转化细胞中生长的一些有包膜RNA病毒的凝集性对刀豆球蛋白A（Con A）浓度的依赖性与在未转化细胞中生长的病毒进行了比较。禽肿瘤病毒通过在常规等密度蔗糖密度梯度离心后，在酒石酸钾和甘油的组合平衡：粘度梯度中离心至平衡来纯化。与在未转化细胞中生长时相比，禽肿瘤病毒在转化细胞中生长时对Con A的凝集性更强。在转化细胞中生长的水疱性口炎病毒也比在未转化细胞中生长的病毒更易凝集。这些结果与以下概念一致，即包膜会被宿主细胞转化所修饰，因此，预计在转化细胞中生长的病毒比在未转化细胞中生长的病毒对Con A的凝集性更强。
