Miki T
Microbiol Immunol. 1981;25(6):585-94. doi: 10.1111/j.1348-0421.1981.tb00060.x.
Electrochemical properties of the glycoprotein of vesicular stomatitis virus (VSV) grown in Rous sarcoma virus (RSV)-transformed cells was compared with that of its counterpart grown in nontransformed cells. In DEAE-Sephadex column chromatography, the glycoproteins of VSV derived from transformed cells appeared more heterogeneous and had a tendency to elute with higher concentrations of NaCl than those from nontransformed cells. In isoelectric focussing, the glycoproteins of VSVs derived from transformed and nontransformed cells appeared as multiple components differing in the isoelectric point, and the glycoproteins from virus from transformed cells had isoelectric points that were more acidic than their counterparts from nontransformed cells. These results show that the glycoprotein of VSV consists of populations of molecules differing in charge and their isoelectric points were shifted to the acidic side by host cell transformation.
将在劳斯肉瘤病毒（RSV）转化细胞中生长的水疱性口炎病毒（VSV）糖蛋白的电化学性质与其在未转化细胞中生长的对应物进行了比较。在DEAE-葡聚糖凝胶柱色谱中，来自转化细胞的VSV糖蛋白显得更加不均一，并且比来自未转化细胞的糖蛋白更倾向于在较高浓度的NaCl中洗脱。在等电聚焦中，来自转化和未转化细胞的VSV糖蛋白表现为等电点不同的多个组分，并且来自转化细胞的病毒的糖蛋白的等电点比来自未转化细胞的对应物更偏酸性。这些结果表明，VSV糖蛋白由电荷不同的分子群体组成，并且它们的等电点通过宿主细胞转化向酸性侧移动。
