Larsen G R, Dorner A J, Harris T J, Wimmer E
Nucleic Acids Res. 1980 Mar 25;8(6):1217-29. doi: 10.1093/nar/8.6.1217.
The structure of polio replicative form (RF) has been investigated by 3' end labeling and the use of polynucleotide phosphorylase to now allow a complete composite of the RF structure. The evidence presented indicates that the 3' terminal sequence of the minus strand is an exact complement to the 5' end of polio RNA. This suggests that the 5' terminal U of polio RNA is genetically coded. Other data is presented to show that in addition to the genetically coded poly(A) tract of the plus strand in RF, a single-stranded poly(A) tail protrudes beyond the double-stranded RNA.
通过3'末端标记和使用多核苷酸磷酸化酶对脊髓灰质炎复制型（RF）的结构进行了研究，现在已得出RF结构的完整组合。所提供的证据表明，负链的3'末端序列与脊髓灰质炎RNA的5'末端完全互补。这表明脊髓灰质炎RNA的5'末端U是由基因编码的。还提供了其他数据以表明，除了RF中正义链的基因编码的多聚腺苷酸序列外，单链多聚腺苷酸尾巴突出于双链RNA之外。
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