Grinenko N F, Tarasishin L A, Valikhov A F, Zajac V, Al'tshteĭn A D
Vopr Virusol. 1980 May-Jun(3):281-4.
A comparative analysis of the results of bovine sera examinations by three methods for the detection of antibody to bovine leukemia virus (BLV): VSV BLV pseudotype neutralization test (PTNT), gel immunodiffusion using BLV glycoprotein antigen (GIDgp) and BLV 125I-p24 radioimmunoprecipitation (RIPp24), was performed. The results of all the three methods were in good correlation. The PTNT sensitivity was shown to exceed essentially all the other methods of BLV antibody detection. There was a definite correlation between the serum titer in PTNT and the possibility of antibody detection in RIPp24 and GIDgp. The potentials of these methods for diagnosis of BLV infection in animals are discussed.
采用三种方法检测牛白血病病毒（BLV）抗体对牛血清进行检测，并进行比较分析：水泡性口炎病毒（VSV）-BLV假型中和试验（PTNT）、使用BLV糖蛋白抗原的凝胶免疫扩散试验（GIDgp）和BLV 125I-p24放射免疫沉淀试验（RIPp24）。所有三种方法的结果具有良好的相关性。结果表明，PTNT的灵敏度基本上超过了所有其他BLV抗体检测方法。PTNT中的血清滴度与RIPp24和GIDgp中抗体检测的可能性之间存在明确的相关性。讨论了这些方法在动物BLV感染诊断中的潜力。
