Zajac V, Altaner C, Závada J, Cerný L
Neoplasma. 1980;27(5):517-23.
Two methods for the detection of antibodies to the bovine leukemia virus (BLV) in infected animals were compared for their suitability for the early diagnosis of bovine leukemia - pseudotype neutralization test (PNT) employing vesicular stomatitis virus - bovine leukemia virus pseudotypes (VSV-BLV), and radioimmunoassay test (RIA) for major internal viral protein p24 of the BLV. The comparison was made using more than 300 sera from cows of the herds with high incidence of bovine leukemia. In infected animals the presence of antibodies against virus envelope glycoprotein detected by PNT and antibodies against major structural viral protein p24 detected by RIA were found always coincidentally. Both methods were found highly comparable and suitable for early detection of bovine leukemia virus infected animals.
比较了两种检测感染动物中牛白血病病毒（BLV）抗体的方法，以评估它们对牛白血病早期诊断的适用性——采用水疱性口炎病毒-牛白血病病毒假型（VSV-BLV）的假型中和试验（PNT），以及针对BLV主要内部病毒蛋白p24的放射免疫测定试验（RIA）。使用来自牛白血病高发牛群的300多头奶牛的血清进行了比较。在感染动物中，通过PNT检测到的针对病毒包膜糖蛋白的抗体与通过RIA检测到的针对主要结构病毒蛋白p24的抗体总是同时存在。发现这两种方法具有高度可比性，适用于早期检测感染牛白血病病毒的动物。
