Giron L T, Crutcher K A, Davis J N
Ann Neurol. 1980 Nov;8(5):520-5. doi: 10.1002/ana.410080509.
Noradrenergic fibers were identified in rat cervical lymph nodes by fluorescence histochemistry and confirmed by radioenzymatic determination of norepinephrine. After superior cervical ganglionectomy, lymph node norepinephrine and noradrenergic fluorescence were strikingly decreased. The alpha-adrenergic radioligand [3H] dihydroergocryptine bound to saturable sites on lymph node membranes (0.20 pmol/mg protein; affinity constant, 8 nM) with characteristics expected for alpha-adrenergic receptor binding. The alpha-adrenergic binding sites were present in high concentration in membranes from lymph node stroma but were negligible in membranes of lymphocytes teased from nodes. In lymph nodes the ratio of alpha-adrenergic binding sites to norepinephrine content was higher than ratios of many other sympathetically innervated tissues such as iris or vas deferens. The presence of sympathetic innervation and the high concentration of alpha-adrenergic binding sites relative to that innervation suggest that the sympathetic nervous system modulates an intrinsic lymph node function through alpha-adrenergic mechanisms.
通过荧光组织化学法在大鼠颈淋巴结中鉴定出去甲肾上腺素能纤维，并通过放射性酶法测定去甲肾上腺素予以证实。颈上神经节切除术后，淋巴结中的去甲肾上腺素和去甲肾上腺素能荧光显著降低。α-肾上腺素能放射性配体[³H]二氢麦角隐亭与淋巴结膜上的可饱和位点结合（0.20 pmol/mg蛋白质；亲和常数，8 nM），具有α-肾上腺素能受体结合预期的特征。α-肾上腺素能结合位点在淋巴结基质膜中浓度很高，但从淋巴结中分离出的淋巴细胞膜中则可忽略不计。在淋巴结中，α-肾上腺素能结合位点与去甲肾上腺素含量的比值高于许多其他受交感神经支配的组织，如虹膜或输精管。交感神经支配的存在以及相对于该神经支配而言α-肾上腺素能结合位点的高浓度表明，交感神经系统通过α-肾上腺素能机制调节淋巴结的内在功能。
