Marquardt W W, Johnson R B, Odenwald W F, Schlotthober B A
Avian Dis. 1980 Apr-Jun;24(2):375-85.
An immuno-enzyme assay for measuring infectious bursal disease antibodies in chickens is described. The test is performed rapidly after coating plates overnight with partially purified antigen prepared in cell culture. Coated plates can be stored for at last 4 months. The chromatographically purified rabbit anti-chicken immunoglobulin-G, conjugated to horseradish peroxidase, was used optimally at a dilution of 1:3,000. It could be stored for at least 10 months without a reduction in titer. The test is safe, highly reproducible, specific, and sensitive. Results can be read visually or by spectrophotometry. Antibodies could be detected as early as 4 days postinfection. Serum titers rose rapidly to high levels, ranging from 1:1,600 to 1:25,600 by one week postinfection. High titers persisted for up to one year. The results of this assay compare favorably with results obtained with the agar-gel precipitin and virus-neutralization tests.
本文描述了一种用于检测鸡传染性法氏囊病抗体的免疫酶测定法。在用细胞培养制备的部分纯化抗体制备板过夜包被后，该测试可快速进行。包被板可储存至少4个月。经色谱纯化的兔抗鸡免疫球蛋白G与辣根过氧化物酶偶联，最佳使用稀释度为1:3000。它可以储存至少10个月而不降低效价。该测试安全、高度可重复、特异且灵敏。结果可以通过目视或分光光度法读取。感染后4天即可检测到抗体。血清效价在感染后一周内迅速上升至高水平，范围从1:1600到1:25600。高效价可持续长达一年。该测定法的结果与琼脂凝胶沉淀试验和病毒中和试验的结果相比具有优势。
