酶联免疫吸附测定法与血凝抑制试验及免疫扩散试验在检测鸡体内针对一种血凝性鸭腺病毒抗体方面的比较。
Comparison of enzyme-linked immunosorbent assay with hemagglutination-inhibition and immunodiffusion tests for detection of antibodies to a hemagglutinating duck adenovirus in chickens.
作者信息
Piela T H, Yates V J
出版信息
Avian Dis. 1983 Jul-Sep;27(3):724-30.
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to a hemagglutinating duck adenovirus is described. Optimum conditions for the test were determined, and the system was compared with procedures currently used. Experimentally infected chickens were assayed for specific antibody by ELISA, hemagglutination inhibition (HI), and immunodiffusion (ID). The ELISA was a sensitive and reliable method for detecting antibody, although positive titers were not always in agreement with HI and ID results at 1 week postinoculation, probably reflecting the different classes of antibody being detected.
描述了一种用于检测抗血凝鸭腺病毒抗体的间接酶联免疫吸附测定（ELISA）方法。确定了该检测的最佳条件，并将该系统与目前使用的方法进行了比较。通过ELISA、血凝抑制（HI）和免疫扩散（ID）对实验感染的鸡进行特异性抗体检测。ELISA是一种检测抗体的灵敏且可靠的方法，尽管在接种后1周时阳性滴度并不总是与HI和ID结果一致，这可能反映了所检测抗体的不同类别。
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