Cho B R, McDonald T L
Avian Dis. 1980 Apr-Jun;24(2):423-34.
Infectious bursal disease virus (IBDV) that had been adapted to grow and was then cloned in chick embryo fibroblast (CEF) cell culture was examined for its physicochemical properties, the cellular site of virus replication, and the nature of its viral RNA. The IBDV was an RNA virus, acid-stable, absolutely resistant to chloroform, and moderately thermolabile. It appeared to replicate only in the cytoplasm, as shown by virus-specific antigens restricted to the cytoplasm of infected cells. The viral RNA was composed of single-stranded RNA, as evidenced by flame-red fluorescence on acridine-orange staining and an absence of specific fluorescence in infected cells on immunofluorescent staining with antiserum specific for double-stranded RNA. The IBDV virion had a hexagonal outline with an average diameter of 62 nm and possessed a single layer of capsid composed of hollow capsomeres without envelope. The buoyant density as determined in a continuous sucrose gradient was 1.178 g/cm3. The IBDV was found to possess morphologial and physicochemical properties different from those of any established RNA virus group.
对已适应生长并随后在鸡胚成纤维细胞（CEF）培养物中克隆的传染性法氏囊病病毒（IBDV）进行了其物理化学性质、病毒复制的细胞位点及其病毒RNA性质的研究。IBDV是一种RNA病毒，耐酸，对氯仿绝对抗性，且中度热不稳定。如感染细胞的细胞质中存在病毒特异性抗原所示，它似乎仅在细胞质中复制。病毒RNA由单链RNA组成，吖啶橙染色呈现火焰红色荧光以及用双链RNA特异性抗血清进行免疫荧光染色时感染细胞中无特异性荧光可证明这一点。IBDV病毒粒子呈六边形轮廓，平均直径为62nm，具有由无包膜的中空衣壳粒组成的单层衣壳。在连续蔗糖梯度中测定的浮力密度为1.178g/cm³。发现IBDV具有与任何已确定的RNA病毒组不同的形态和物理化学性质。
