Strumpf I J, Tsang A Y, Schork M A, Weg J G
Am Rev Respir Dis. 1976 Nov;114(5):971-6. doi: 10.1164/arrd.1976.114.5.971.
From 1972 to 1974, all sputum specimens and gastric aspirate specimens submitted to the University of Michigan Laboratory for acid-fast smear and culture were studied. Specimens were paired for culture and smear results using the auramine-rhodamine staining technique. Of 1,893 patients, 75 patients without prior antituberculous therapy were found to have either a positive smear or a positive culture of either sputum or gastric material. The data analyzed by patient source revealed the following. (1) Staining sputum with auramine-rhodamine is a clinically reliable technique for detecting pulmonary tuberculosis. It demonstrates a sensitivity of 78 per cent and a relative fraction of false positive smears of only 11 per cent. (2) Staining gastric-aspirated material by the auramine-rhodamine technique is not a clinically reliable method as a routine procedure for the detection of pulmonary tuberculosis, because of a sensitivity of only 58.8 per cent and a relative fraction of false-positive smears of 33 per cent. (3) In the absence of sputum in suspected clinical granulomatous disease, quantified gastric smears may be helpful. In this study, when more than 6 organisms per high power field were found, the patient's sputum or gastric material yielded a pathogenic mycobacterium on culture.
1972年至1974年期间，对所有提交至密歇根大学实验室进行抗酸涂片和培养的痰液标本及胃抽吸物标本进行了研究。使用金胺 - 罗丹明染色技术对标本的培养和涂片结果进行配对。在1893例患者中，发现75例未接受过抗结核治疗的患者痰液或胃内容物涂片或培养呈阳性。按患者来源分析的数据显示如下：（1）用金胺 - 罗丹明对痰液进行染色是检测肺结核的一种临床可靠技术。其灵敏度为78%，假阳性涂片的相对比例仅为11%。（2）用金胺 - 罗丹明技术对胃抽吸物进行染色作为检测肺结核的常规程序并非临床可靠方法，因为其灵敏度仅为58.8%，假阳性涂片的相对比例为33%。（3）在疑似临床肉芽肿病且无痰液的情况下，定量胃涂片可能会有帮助。在本研究中，当每高倍视野发现超过6个菌体时，患者的痰液或胃内容物培养出致病性分枝杆菌。
