Strumpf I J, Tsang A Y, Sayre J W
Am Rev Respir Dis. 1979 Apr;119(4):599-602. doi: 10.1164/arrd.1979.119.4.599.
Data were collected at 2 teaching institutions, University Hospital, University of Michigan (UMH) and University of California at Los Angeles (UCLA) Hospital, to evaluate the usefulness of the auramine-rhodamine fluorochrome stain in the diagnosis of pulmonary tuberculosis in hospitalized patients. The patients studied had received no prior therapy, and their sputum specimens were positive for a pathogenic Mycobacterium by microscopy or culture or both. The true-positive smear rate was 0.88 at UMH and 0.83 at UCLA; the relative false-positive smear rate was 0.12 at UMH and 0.17 at UCLA. The sensitivities at UMH and UCLA were 0.78 and 0.51, respectively, and the specificity was greater than 0.99 at both. The data suggest that sputum stained by the fluorochrome method is useful and reliable for the diagnosis of pulmonary tuberculosis, because the false-positive rates are acceptably low in hospitalized patients.
在密歇根大学大学医院（UMH）和加利福尼亚大学洛杉矶分校（UCLA）医院这两家教学机构收集数据，以评估金胺 - 罗丹明荧光染色法在诊断住院患者肺结核中的实用性。所研究的患者此前未接受过治疗，且他们的痰标本通过显微镜检查或培养或两者均显示致病性分枝杆菌呈阳性。UMH的真阳性涂片率为0.88，UCLA为0.83；UMH的相对假阳性涂片率为0.12，UCLA为0.17。UMH和UCLA的敏感度分别为0.78和0.51，两者的特异度均大于0.99。数据表明，荧光染色法染色的痰对于肺结核的诊断是有用且可靠的，因为在住院患者中假阳性率低到可以接受。
