用甲酰胺解离脱氧核糖核酸酶I.肌动蛋白复合物

Dissociation of the DNAse-I . actin complex by formamide.

作者信息

Zechel K

出版信息

Eur J Biochem. 1980 Sep;110(2):337-41. doi: 10.1111/j.1432-1033.1980.tb04872.x.

DOI:10.1111/j.1432-1033.1980.tb04872.x

Abstract

Rabbit skeletal muscle actin labeled with 125 iodine by an enzymic method is shown to be capable of polymerization and to bind to matrix-bound pancreatic DNAse I like unlabeled G-actin. It was used to demonstrate that actin can be released from DNAse-I-agarose by 35--40% formamide. Actin which was only shortly exposed to this solvent was able to bind again to DNAse I and to form filaments indicating that it has been recovered functionally intact from the affinity matrix.

摘要

通过酶法用¹²⁵碘标记的兔骨骼肌肌动蛋白，显示出能够聚合，并像未标记的G-肌动蛋白一样与结合在基质上的胰腺DNA酶I结合。它被用于证明肌动蛋白可以被35%-40%的甲酰胺从DNA酶I-琼脂糖中释放出来。仅短暂暴露于这种溶剂的肌动蛋白能够再次与DNA酶I结合并形成细丝，这表明它已从亲和基质中功能性完整地回收。