Couturier J, Lejeune J
Ann Genet. 1976 Sep;19(3):217-8.
Acridine orange, coriphosphine, toluidin blue, or pyronin, when added to the hypotonic solution, allow G-banding when the fixed slides are stained in the usual way. Apart from this simple practical application, these modifications of chromatid structure by a treatment applied before fixation shed some light on the molecular mechanisms responsible for chromosome banding.
吖啶橙、金精三羧酸、甲苯胺蓝或派洛宁添加到低渗溶液中后,常规方法染色固定玻片时可实现G显带。除了这一简单的实际应用外,固定前进行的这种染色单体结构修饰处理,为染色体显带的分子机制提供了一些线索。