Raghavan N, Ishaq M, Kaji A
J Virol. 1980 Aug;35(2):551-4. doi: 10.1128/JVI.35.2.551-554.1980.
Rts1 is a plasmid which confers upon the host bacteria the capacity to restrict T4 bacteriophage growth at 32 degrees C but not at 42 degrees C. Pulse-labeling of phage-infected cells showed that Rts1 restricts the synthesis of T1 DNA. Despite efficient restriction of T4 phage growth and DNA synthesis, infected Escherichia coli 20SO harboring Rts1 synthesized both early and late T4 phage RNA. Synthesis of early T4 phage RNA under restrictive conditions (32 degrees C) was almost equal to that found under nonrestrictive conditions, and a lesser, but significant, amount of late T4 phage RNA was made in almost complete absence of T4 DNA synthesis. Moreover, very little, if any, T4 phage-coded lysozyme was detected in the infected E. coli 20SO/Rts1 at 32 degrees C, whereas normal amounts of lysozyme were present at 42 degrees C.
Rts1是一种质粒，它赋予宿主细菌在32℃时限制T4噬菌体生长的能力，但在42℃时则没有这种能力。对噬菌体感染细胞进行脉冲标记显示，Rts1限制T4 DNA的合成。尽管T4噬菌体的生长和DNA合成受到有效限制，但携带Rts1的受感染大肠杆菌20SO合成了早期和晚期T4噬菌体RNA。在限制性条件（32℃）下早期T4噬菌体RNA的合成几乎与非限制性条件下的合成量相等，并且在几乎完全没有T4 DNA合成的情况下，仍合成了少量但显著量的晚期T4噬菌体RNA。此外，在32℃时，在受感染的大肠杆菌20SO/Rts1中几乎检测不到T4噬菌体编码的溶菌酶，而在42℃时则存在正常量的溶菌酶。
