Ascoli M
J Biol Chem. 1981 Jan 10;256(1):179-83.
The MA-10 line is a clonal strain of Leydig tumor cells that has receptors for human choriogonadotropin (hCG) and mouse epidermal growth factor (mEGF). These cells respond to hCG, cholera toxin, and 8-Br-adenosine 3':5'-monophosphate with increased steroid production. It is reported herein that exposure of the MA-10 cells to mEGF results in a substantial (80 to 90%) reduction in the number of hCG receptors per cell. The loss of hCG receptors is accompanied by a corresponding reduction in the ability of hCG to stimulate steroidogenesis. The steroidogenic responses to cholera toxin and 8-Br-adenosine 3':5'-monophosphate, however, are not affected. Other results presented show that mEGF is not a mitogen for these cells.
MA-10细胞系是一种莱迪希肿瘤细胞的克隆株，它具有人绒毛膜促性腺激素（hCG）和小鼠表皮生长因子（mEGF）的受体。这些细胞对hCG、霍乱毒素和8-溴腺苷3':5'-单磷酸的反应是类固醇生成增加。本文报道，MA-10细胞暴露于mEGF会导致每个细胞的hCG受体数量大幅减少（80%至90%）。hCG受体的丧失伴随着hCG刺激类固醇生成能力的相应降低。然而，对霍乱毒素和8-溴腺苷3':5'-单磷酸的类固醇生成反应不受影响。所呈现的其他结果表明，mEGF对这些细胞不是有丝分裂原。
