关于培养的睾丸间质细胞瘤细胞中人类绒毛膜促性腺激素和小鼠表皮生长因子细胞表面受体调节所涉及的机制
On the mechanisms involved in the regulation of the cell-surface receptors for human choriogonadotropin and mouse epidermal growth factor in cultured Leydig tumor cells.
作者信息
Lloyd C E, Ascoli M
出版信息
J Cell Biol. 1983 Feb;96(2):521-6. doi: 10.1083/jcb.96.2.521.
Abstract
The MA-10 cells are a clonal strain of mouse Leydig tumor cells that have receptors for human choriogonadotropin (hCG) and mouse epidermal growth factor (mEGF). Exposure of the cells to hCG results in a reduction in the number of surface hCG receptors, and little or no change in the number of surface mEGF receptors. On the other hand, exposure of the cells to mEGF results in a reduction in the number of both surface mEGF receptors and surface hCG receptors. In order to study these phenomena, we assumed that the number of surface receptors is determined by the rate at which receptors appear at the surface and by the rate of receptor internalization. When these rates were measured, we found that hCG and mEGF reduce their respective surface receptors by increasing the rate of receptor internalization, and that mEGF reduces the surface hCG receptors by decreasing the rate of appearance of the receptor.
摘要
MA-10细胞是小鼠睾丸间质细胞瘤的克隆株，具有人绒毛膜促性腺激素（hCG）和小鼠表皮生长因子（mEGF）的受体。将细胞暴露于hCG会导致表面hCG受体数量减少，而表面mEGF受体数量几乎没有变化或没有变化。另一方面，将细胞暴露于mEGF会导致表面mEGF受体和表面hCG受体数量均减少。为了研究这些现象，我们假设表面受体的数量由受体出现在表面的速率和受体内化速率决定。当测量这些速率时，我们发现hCG和mEGF通过增加受体内化速率来减少它们各自的表面受体，并且mEGF通过降低受体出现的速率来减少表面hCG受体。
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Insulin-induced down-regulation of insulin receptors in 3T3-L1 adipocytes. Altered rate of receptor inactivation.胰岛素诱导3T3-L1脂肪细胞中胰岛素受体的下调。受体失活速率改变。
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Desensitization of steroidogenesis in cultured Leydig tumor cells: role of cholesterol.培养的睾丸间质细胞瘤细胞中类固醇生成的脱敏作用：胆固醇的作用
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Internalization and degradation of receptor-bound human choriogonadotropin in Leydig tumor cells. Fate of the hormone subunits.莱迪希肿瘤细胞中受体结合型人绒毛膜促性腺激素的内化与降解。激素亚基的命运。
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