Myers M W, Carter B J
J Biol Chem. 1981 Jan 25;256(2):567-70.
We have studied the relationship between adeno-associated virus (AAV) DNA replication and virus particle assembly. Formation of empty or full particles and accumulation of AAV capsid proteins was prevented in the presence of the arginine analogue, L-canavanine, or when a temperature-sensitive helper adenovirus was used at the nonpermissive temperature. In each case there was a concomitant inhibition of AAV single-stranded (progeny) DNA accumulation but little or no effect upon synthesis of AAV duplex, replicating form DNA. These results indicate that AAV protein, perhaps in the form of assembled capsids, is required for AAV single-stranded progeny DNA accumulation.
我们研究了腺相关病毒（AAV）DNA复制与病毒颗粒组装之间的关系。在精氨酸类似物L-刀豆氨酸存在的情况下，或者当使用温度敏感型辅助腺病毒在非允许温度下时，空颗粒或完整颗粒的形成以及AAV衣壳蛋白的积累受到抑制。在每种情况下，AAV单链（子代）DNA的积累都受到了相应的抑制，但对AAV双链复制型DNA的合成几乎没有影响。这些结果表明，AAV子代单链DNA的积累需要AAV蛋白，可能是以组装好的衣壳形式存在。
