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腺病毒E1B 55千道尔顿多肽促进腺相关病毒mRNA及时在细胞质中积累。

Adenovirus E1B 55-Mr polypeptide facilitates timely cytoplasmic accumulation of adeno-associated virus mRNAs.

作者信息

Samulski R J, Shenk T

机构信息

Department of Molecular Biology, Princeton University, New Jersey 08544.

出版信息

J Virol. 1988 Jan;62(1):206-10. doi: 10.1128/JVI.62.1.206-210.1988.

DOI:10.1128/JVI.62.1.206-210.1988
PMID:2824848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC250520/
Abstract

Adenovirus provides helper functions that facilitate replication of adeno-associated virus (AAV). Both the adenovirus E1B 55-Mr and E4 34-Mr polypeptides are required for efficient and timely accumulation of AAV mRNA, proteins, and DNA. The E1B 55-Mr polypeptide is also required for rescue of the integrated AAV genome in Detroit 6-D5 cells in a normal time frame. All of these effects probably result from a single, primary delay in AAV mRNA accumulation. The AAV helper function provided by the E1B 55-Mr and E4 34-Mr polypeptides appears to closely parallel their normal role in the adenovirus replication cycle.

摘要

腺病毒提供辅助功能，促进腺相关病毒（AAV）的复制。腺病毒E1B 55-kDa和E4 34-kDa多肽对于AAV mRNA、蛋白质和DNA的高效及时积累都是必需的。E1B 55-kDa多肽对于在正常时间框架内拯救底特律6-D5细胞中整合的AAV基因组也是必需的。所有这些效应可能都源于AAV mRNA积累中的单一主要延迟。E1B 55-kDa和E4 34-kDa多肽提供的AAV辅助功能似乎与其在腺病毒复制周期中的正常作用密切平行。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/35eba9a834d6/jvirol00080-0229-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/35eba9a834d6/jvirol00080-0229-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/18c9fe1b6046/jvirol00080-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/5d7de3b6a2b1/jvirol00080-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/0c24d0483c8f/jvirol00080-0228-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1945/250520/35eba9a834d6/jvirol00080-0229-b.jpg

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A monoclonal antibody detecting the adenovirus type 5-E1b-58Kd tumor antigen: characterization of the E1b-58Kd tumor antigen in adenovirus-infected and -transformed cells.一种检测腺病毒5型-E1b-58Kd肿瘤抗原的单克隆抗体：腺病毒感染和转化细胞中E1b-58Kd肿瘤抗原的特性
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Functional analysis of adenovirus-5 host-range deletion mutants defective for transformation of rat embryo cells.
AAV2 可以通过滚动发夹或滚动环机制复制其 DNA，具体取决于辅助病毒。
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Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells.影响 HEK-293 细胞中三质粒瞬时转染时 rAAV 滴度的因素。
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The Interplay between Adeno-Associated Virus and its Helper Viruses.腺相关病毒与其辅助病毒之间的相互作用。
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Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction.腺相关病毒基因组相互作用对载体生产和转导很重要。
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Herpes simplex virus types 1 and 2 completely help adenovirus-associated virus replication.1型和2型单纯疱疹病毒完全有助于腺病毒相关病毒的复制。
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