Cumming R
J Immunol Methods. 1980;37(3-4):301-9. doi: 10.1016/0022-1759(80)90315-4.
Radioimmunoassay techniques have been used to determine why only a small number of antibodies against cyclic GMP are able to show immunofluorescent staining in the central nervous system. However, neither the titre, avidity nor specificity as determined in liquid-phase radioimmunoassay employing [3H]cyclic GMP, have been able to predict whether a given antibody will show specific staining, or account for the different sites of immunofluorescent localisation observed with these antibodies. These observations are discussed in terms of the populations of antibody molecules employed in the radioimmunoassay and immunofluorescent procedures.
放射免疫分析技术已被用于确定为何仅有少量抗环鸟苷酸（cGMP）抗体能够在中枢神经系统中显示免疫荧光染色。然而，在使用[3H]环鸟苷酸的液相放射免疫分析中所测定的效价、亲和力或特异性，均无法预测某一特定抗体是否会显示特异性染色，也无法解释用这些抗体观察到的免疫荧光定位的不同部位。本文根据放射免疫分析和免疫荧光检测中所使用的抗体分子群体对这些观察结果进行了讨论。
