通过酵母中的互补作用从果蝇中分离出一个基因。
Isolation of a gene from Drosophila by complementation in yeast.
作者信息
Henikoff S, Tatchell K, Hall B D, Nasmyth K A
出版信息
Nature. 1981 Jan 1;289(5793):33-7. doi: 10.1038/289033a0.
DOI:10.1038/289033a0
PMID:6256646
Abstract
Transformation of mutant yeast cells by cloned genomic DNA from a higher eukaryote has made it possible to isolate a Drosophila DNA sequence that complements a yeast adenine-8-mutation. A 0.8-kilobase poly(A)-containing RNA is transcribed from the cloned Drosophila segment in transformed yeast cells and can account for functional expression of the gene.
摘要
用来自高等真核生物的克隆基因组DNA对突变酵母细胞进行转化，使得分离出一个能互补酵母腺嘌呤-8-突变的果蝇DNA序列成为可能。在转化的酵母细胞中，从克隆的果蝇片段转录出一种含0.8千碱基的聚腺苷酸RNA，它可以解释该基因的功能表达。
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