Gerencser G A
Am J Physiol. 1981 Jan;240(1):R61-9. doi: 10.1152/ajpregu.1981.240.1.R61.
This investigation was principally undertaken to examine the mechanism by which organic solutes (amino acids) stimulate chloride transport across the Aplysia californica intestine. Isolated intestine, mounted between identical oxygenated seawater solutions, maintained stable transmural potential differences (serosa negative) and short-circuit currents for several hours at 25 degrees C. The addition of glycine to the mucosal solution stimulated rapid sustained increases in these electrical characteristics. The change in short-circuit increased curvilinearly with increasing concentrations of mucosal glycine. Mucosal glycine stimulated transmural potential difference and short-circuit current after mucosal phlorizin had partially inhibited D-glucose stimulation of the electrical characteristics. Mucosal glycine enhanced the transmural electrical characteristics. Mucosal glycine enhanced the transmural electrical characteristics after serosal ouabain had abolished them. The major portion of the amino acid-induced short-circuit current was carried by a net, active, chloride transfer from mucosa to serosa as determined by flux measurements. These results suggest that the amino acid-induced effect on chloride transport is mediated by a common mucosal membrane carrier for both sodium and the amino acid.
本研究主要旨在探讨有机溶质（氨基酸）刺激加州海兔肠道氯离子转运的机制。将离体肠道置于相同的充氧海水溶液之间，在25℃下可维持数小时稳定的跨壁电位差（浆膜侧为负）和短路电流。向黏膜溶液中添加甘氨酸会刺激这些电学特性迅速持续增加。短路电流的变化随黏膜甘氨酸浓度的增加呈曲线增加。在黏膜根皮苷部分抑制D - 葡萄糖对电学特性的刺激后，黏膜甘氨酸仍能刺激跨壁电位差和短路电流。黏膜甘氨酸增强了跨壁电学特性。在浆膜侧哇巴因消除跨壁电学特性后，黏膜甘氨酸仍能增强其特性。通过通量测量确定，氨基酸诱导的短路电流的主要部分是由氯离子从黏膜到浆膜的净主动转运所携带。这些结果表明，氨基酸对氯离子转运的诱导作用是由一种同时转运钠和氨基酸的共同黏膜膜载体介导的。
