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New method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests.

作者信息

Chojkier M, Peterkofsky B, Bateman J

出版信息

Anal Biochem. 1980 Nov 1;108(2):385-93. doi: 10.1016/0003-2697(80)90603-x.

DOI:10.1016/0003-2697(80)90603-x
PMID:6257145
Abstract
摘要

相似文献

1
New method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests.通过测量胶原酶消化物中[4-³H]:[¹⁴C]脯氨酸比例的变化来测定脯氨酸羟化程度的新方法。
Anal Biochem. 1980 Nov 1;108(2):385-93. doi: 10.1016/0003-2697(80)90603-x.
2
[3H]tryptophan-[14C]proline dual label method for the simultaneous determination of collagen and noncollagen protein production.
Anal Biochem. 1984 Nov 1;142(2):386-94. doi: 10.1016/0003-2697(84)90481-0.
3
A [4,5-3H]lysine:[14C]lysine dual-label method to measure lysine hydroxylation in collagen.一种用于测量胶原蛋白中赖氨酸羟基化的[4,5-³H]赖氨酸:[¹⁴C]赖氨酸双标记方法。
Anal Biochem. 1986 Oct;158(1):151-7. doi: 10.1016/0003-2697(86)90603-2.
4
Comprehensive analysis of collagen metabolism in vitro using [4(3H)]/[14C]proline dual-labeling and polyacrylamide gel electrophoresis.使用[4(³H)]/[¹⁴C]脯氨酸双标记和聚丙烯酰胺凝胶电泳对体外胶原蛋白代谢进行综合分析。
Anal Biochem. 1988 Jan;168(1):171-5. doi: 10.1016/0003-2697(88)90025-5.
5
Age-related variations in hydroxylation of lysine and proline in collagen.胶原蛋白中赖氨酸和脯氨酸羟基化的年龄相关变化。
Biochem J. 1974 May;139(2):461-8. doi: 10.1042/bj1390461.
6
[Biosynthesis and properties of collagen forming in the cartilage of chick embryos in the presence of various proline analogs].[在各种脯氨酸类似物存在的情况下鸡胚软骨中形成的胶原蛋白的生物合成及特性]
Vopr Med Khim. 1976 Nov-Dec;22(6):830-4.
7
Effect of cortisone on collagenolytic activity in the rat.可的松对大鼠胶原溶解活性的影响。
Proc Soc Exp Biol Med. 1975 Mar;148(3):887-90. doi: 10.3181/00379727-148-38654.
8
Biosynthesis of collagen (14C)hydroxyproline and (14C)hydroxylysine during maturation of chick embryos.鸡胚成熟过程中胶原蛋白、(14C)羟脯氨酸和(14C)羟赖氨酸的生物合成。
Mech Ageing Dev. 1973 Mar;1(5):445-50. doi: 10.1016/0047-6374(73)90050-x.
9
Studies in vivo on the biosynthesis of collagen and elastin in ascorbic acid-deficient guinea pigs.关于抗坏血酸缺乏的豚鼠体内胶原蛋白和弹性蛋白生物合成的研究。
Biochem J. 1969 Jun;113(2):387-97. doi: 10.1042/bj1130387.
10
Ascorbate-dependent differences in the hydroxylation of proline and lysine in collagen synthesized by 3T6 fibroblasts in culture.培养的3T6成纤维细胞合成的胶原蛋白中脯氨酸和赖氨酸羟基化过程中依赖抗坏血酸的差异。
Biochim Biophys Acta. 1972 Oct 31;278(3):610-6. doi: 10.1016/0005-2795(72)90025-6.

引用本文的文献

1
Abnormal type I collagen metabolism by cultured fibroblasts in lethal perinatal osteogenesis imperfecta.致死性围生期成骨不全中培养的成纤维细胞的I型胶原代谢异常。
Biochem J. 1984 Jan 1;217(1):103-15. doi: 10.1042/bj2170103.
2
Specifically decreased collagen biosynthesis in scurvy dissociated from an effect on proline hydroxylation and correlated with body weight loss. In vitro studies in guinea pig calvarial bones.坏血病中胶原蛋白生物合成的特异性降低与脯氨酸羟化作用无关,而是与体重减轻相关。豚鼠颅骨的体外研究。
J Clin Invest. 1983 Sep;72(3):826-35. doi: 10.1172/JCI111053.
3
Mechanisms of Kirsten murine sarcoma virus transformation-induced changes in the collagen phenotype and synthetic rate of BALB 3T3 cells.
柯斯顿鼠肉瘤病毒转化诱导BALB 3T3细胞胶原蛋白表型和合成率变化的机制。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6028-32. doi: 10.1073/pnas.78.10.6028.
4
Hepatocyte collagen production in vivo in normal rats.正常大鼠体内肝细胞胶原蛋白的产生
J Clin Invest. 1986 Aug;78(2):333-9. doi: 10.1172/JCI112581.
5
Introduction of the human pro alpha 1(I) collagen gene into pro alpha 1(I)-deficient Mov-13 mouse cells leads to formation of functional mouse-human hybrid type I collagen.将人类原α1(I)胶原蛋白基因导入缺乏原α1(I)的Mov-13小鼠细胞中,可导致功能性小鼠-人类杂合I型胶原蛋白的形成。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):764-8. doi: 10.1073/pnas.84.3.764.
6
Inhibition of collagen hydroxylation by 2,7,8-trihydroxyanthraquinone in embryonic-chick tendon cells.2,7,8-三羟基蒽醌对鸡胚肌腱细胞中胶原蛋白羟基化的抑制作用。
Biochem J. 1989 Jul 1;261(1):127-30. doi: 10.1042/bj2610127.
7
d-alpha-tocopherol inhibits collagen alpha 1(I) gene expression in cultured human fibroblasts. Modulation of constitutive collagen gene expression by lipid peroxidation.d-α-生育酚抑制培养的人成纤维细胞中胶原蛋白α1(I)基因的表达。脂质过氧化对组成型胶原蛋白基因表达的调节。
J Clin Invest. 1991 Jun;87(6):2230-5. doi: 10.1172/JCI115258.
8
Inhibition of prolyl hydroxylation and procollagen processing in chick-embryo calvaria by a derivative of pyridine-2,4-dicarboxylate. Characterization of the diethyl ester as a proinhibitor.吡啶-2,4-二羧酸衍生物对鸡胚颅骨中脯氨酰羟化作用和前胶原加工的抑制作用。二乙酯作为前抑制剂的特性。
Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):469-76. doi: 10.1042/bj2750469.
9
Intracellular cAMP determines the extent of degradation and not the synthesis of collagen by rat hepatocytes.细胞内的环磷酸腺苷(cAMP)决定大鼠肝细胞胶原蛋白的降解程度,而非合成程度。
Mol Cell Biochem. 1992 Jan 15;109(1):89-94. doi: 10.1007/BF00230878.