通过测量胶原酶消化物中[4-³H]：[¹⁴C]脯氨酸比例的变化来测定脯氨酸羟化程度的新方法。 - Suppr

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超能文献

New method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests.

作者信息

Chojkier M, Peterkofsky B, Bateman J

出版信息

Anal Biochem. 1980 Nov 1;108(2):385-93. doi: 10.1016/0003-2697(80)90603-x.

DOI:10.1016/0003-2697(80)90603-x

PMID:6257145

Abstract

摘要

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New method for determining the extent of proline hydroxylation by measuring changes in the ratio of [4-3H]:[14C]proline in collagenase digests.通过测量胶原酶消化物中[4-³H]：[¹⁴C]脯氨酸比例的变化来测定脯氨酸羟化程度的新方法。

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[Biosynthesis and properties of collagen forming in the cartilage of chick embryos in the presence of various proline analogs].[在各种脯氨酸类似物存在的情况下鸡胚软骨中形成的胶原蛋白的生物合成及特性]

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Biosynthesis of collagen (14C)hydroxyproline and (14C)hydroxylysine during maturation of chick embryos.鸡胚成熟过程中胶原蛋白、（14C）羟脯氨酸和（14C）羟赖氨酸的生物合成。

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Studies in vivo on the biosynthesis of collagen and elastin in ascorbic acid-deficient guinea pigs.关于抗坏血酸缺乏的豚鼠体内胶原蛋白和弹性蛋白生物合成的研究。

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Ascorbate-dependent differences in the hydroxylation of proline and lysine in collagen synthesized by 3T6 fibroblasts in culture.培养的3T6成纤维细胞合成的胶原蛋白中脯氨酸和赖氨酸羟基化过程中依赖抗坏血酸的差异。

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Specifically decreased collagen biosynthesis in scurvy dissociated from an effect on proline hydroxylation and correlated with body weight loss. In vitro studies in guinea pig calvarial bones.坏血病中胶原蛋白生物合成的特异性降低与脯氨酸羟化作用无关，而是与体重减轻相关。豚鼠颅骨的体外研究。

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Proc Natl Acad Sci U S A. 1981 Oct;78(10):6028-32. doi: 10.1073/pnas.78.10.6028.

Hepatocyte collagen production in vivo in normal rats.正常大鼠体内肝细胞胶原蛋白的产生

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Inhibition of collagen hydroxylation by 2,7,8-trihydroxyanthraquinone in embryonic-chick tendon cells.2,7,8-三羟基蒽醌对鸡胚肌腱细胞中胶原蛋白羟基化的抑制作用。

Biochem J. 1989 Jul 1;261(1):127-30. doi: 10.1042/bj2610127.

d-alpha-tocopherol inhibits collagen alpha 1(I) gene expression in cultured human fibroblasts. Modulation of constitutive collagen gene expression by lipid peroxidation.d-α-生育酚抑制培养的人成纤维细胞中胶原蛋白α1（I）基因的表达。脂质过氧化对组成型胶原蛋白基因表达的调节。

J Clin Invest. 1991 Jun;87(6):2230-5. doi: 10.1172/JCI115258.

Inhibition of prolyl hydroxylation and procollagen processing in chick-embryo calvaria by a derivative of pyridine-2,4-dicarboxylate. Characterization of the diethyl ester as a proinhibitor.吡啶-2,4-二羧酸衍生物对鸡胚颅骨中脯氨酰羟化作用和前胶原加工的抑制作用。二乙酯作为前抑制剂的特性。

Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):469-76. doi: 10.1042/bj2750469.

Intracellular cAMP determines the extent of degradation and not the synthesis of collagen by rat hepatocytes.细胞内的环磷酸腺苷（cAMP）决定大鼠肝细胞胶原蛋白的降解程度，而非合成程度。

Mol Cell Biochem. 1992 Jan 15;109(1):89-94. doi: 10.1007/BF00230878.

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