Landry E F, Vaughn J M, Penello W F
Appl Environ Microbiol. 1980 Dec;40(6):1032-8. doi: 10.1128/aem.40.6.1032-1038.1980.
The adsorption rate of a guanidine-resistant strain of poliovirus LSc 2ab was measured in Long Island soils with in situ field cores (10.1 by 75 cm). The test virus was chosen because it exhibited soil adsorption and elution characteristics of a number of non-polioviruses. After the inoculation of cores with seeded sewage effluent at a 1-cm/h infiltration rate, cores were extracted, fractionated, and analyzed for total plaque-forming units per each 5-cm fraction. The results showed that 77% of the viruses were adsorbed in the first 5 cm of soil. An additional 11% were found in the 5- to 10-cm fraction, and a total of 96% of the viruses were adsorbed by 25 cm. The remaining 4% were uniformly distributed over the next 50 cm of soil, with a minimum of 0.23% in each soil section. Few viruses (< 0.22%) were observed in core filtrates. Analysis of the viral distribution pattern in seeded cores, after an application of a single rinse of either sewage effluent or rainwater, indicated that large-scale viral mobilization was absent. However, localized areas of viral movement were noted in both of the rinsed cores, with the rainwater-rinsed cores exhibiting more expensive movement. All mobilized viruses were resorbed at lower core depths.
在长岛土壤中，使用原位田间土柱（10.1×75厘米）测定了一株抗胍脊髓灰质炎病毒LSc 2ab的吸附率。选择该测试病毒是因为它表现出许多非脊髓灰质炎病毒的土壤吸附和洗脱特性。以1厘米/小时的渗透速率用接种了种子污水的土柱进行接种后，对土柱进行提取、分级，并分析每5厘米部分的总空斑形成单位。结果表明，77%的病毒吸附在土壤的前5厘米处。在5至10厘米部分又发现了11%，总共96%的病毒在25厘米处被吸附。其余4%均匀分布在接下来的50厘米土壤中，每个土壤段最少为0.23%。在土柱滤液中观察到的病毒很少（<0.22%）。对接种了种子的土柱在单次用污水或雨水冲洗后的病毒分布模式分析表明，不存在大规模的病毒迁移。然而，在两个冲洗过的土柱中都注意到了局部的病毒移动区域，雨水冲洗过的土柱表现出更广泛的移动。所有迁移的病毒都在较低的土柱深度处重新被吸附。
