Jakubowski W, Hill W F, Clarke N A
Appl Microbiol. 1975 Jul;30(1):58-65. doi: 10.1128/am.30.1.58-65.1975.
Four microporous virus-absorbent filter media for recovering low levels of virus from 380 liters of drinking water were compared. In addition two of the filter media were compared with 1,900 liters of drinking water. The filter media evaluated were MF nitrocellulose membranes (293 mm), AA Cox M-780 epoxy-fiberglass-asbestos disks (267 mm), K-27 yarn-wound fiberglass cartridges + AA Cox M-780 disks (127 mm), and Balston epoxy-fiberglass tubes (24.5 by 63.5 mm). The filters were used to concentrate seeded poliovirus from 380 liters of finished drinking water. Sodium thiosulfate was added to the drinking water to neutralize chlorine, and hydrochloric acid was added to adjust the pH to 3.5. Virus was eluted from the filters with glycine-NaOH buffer at pH 11.5. In terms of virus recovery efficiency, the filter media ranked Balston greater than Cox 267-mm greater than MF 293-mm congruent to K-27 + Cox 127-mm, but differences were slight. The Balston filters and holders were also superior to the other systems in terms of size, weight, cost, and handling factors. Experiments with 2- and 8-mum porosity Balston filters showed no statistically significant difference in virus recovery. Virus was readily detected by the Balston and the MF 293-mm systems at input levels of 12 to 22 PFU/1,900 liters. Preliminary experiments indicated that an elution pH lower than 11.5 may be satisfactory.
比较了四种用于从380升饮用水中回收低水平病毒的微孔病毒吸附过滤介质。此外，还将其中两种过滤介质与1900升饮用水进行了比较。所评估的过滤介质有MF硝酸纤维素膜（293毫米）、AA考克斯M - 780环氧 - 玻璃纤维 - 石棉盘（267毫米）、K - 27缠绕玻璃纤维滤芯 + AA考克斯M - 780盘（127毫米）以及鲍尔斯顿环氧 - 玻璃纤维管（24.5×63.5毫米）。这些过滤器用于从380升成品饮用水中浓缩接种的脊髓灰质炎病毒。向饮用水中添加硫代硫酸钠以中和氯，并添加盐酸将pH值调节至3.5。用pH值为11.5的甘氨酸 - 氢氧化钠缓冲液从过滤器上洗脱病毒。就病毒回收效率而言，过滤介质的排名为鲍尔斯顿大于考克斯267毫米大于MF 293毫米等于K - 27 + 考克斯127毫米，但差异很小。鲍尔斯顿过滤器及其支架在尺寸、重量、成本和操作因素方面也优于其他系统。对孔隙率为2微米和8微米鲍尔斯顿过滤器的实验表明，病毒回收在统计学上无显著差异。在输入水平为12至22 PFU/1900升时，鲍尔斯顿和MF 293毫米系统能够轻松检测到病毒。初步实验表明，洗脱pH值低于11.5可能是令人满意的。
