Tokarskaia O N, Ivanov P L, Markusheva T V, Ryskov A P
Genetika. 1980;16(10):1741-52.
Clones of total mouse DNA efficiently hybridized with mRNA (or cDNA) were selected by colony hybridization technique. The majority of selected fragments demonstrate hybridization with cDNA, dsRNA-B (isolated from pre-mRNA) and oligo(dT). The data obtained indicate that the base sequences hybridizing to these test-probes are contiguous within several individual cloned restriction DNA fragments. At least in two cases sequences hybridizing with cDNA belong to repetitive fraction of the mouse genome (presumptive repetitive structural genes). They are transcribed effectively, and respective mRNAs of abundant type. Two other clones contain structural genes which are expressed into mRNAs of non-abundant type.
通过菌落杂交技术筛选出了能与小鼠总DNA高效杂交的mRNA（或cDNA）克隆。大多数筛选出的片段与cDNA、dsRNA - B（从pre - mRNA中分离得到）和寡聚（dT）杂交。所得数据表明，与这些测试探针杂交的碱基序列在几个单独克隆的限制性DNA片段内是连续的。至少在两种情况下，与cDNA杂交的序列属于小鼠基因组的重复部分（推测为重复结构基因）。它们被有效转录，并产生丰富类型的相应mRNA。另外两个克隆含有可表达为非丰富类型mRNA的结构基因。
