Yamagata H, Nakamura K, Inouye M
J Biol Chem. 1981 Mar 10;256(5):2194-8.
A DNA sequence of 816 base pairs encompassing the entire Erwinia amylovora lipoprotein gene was determined. Sequence comparison between E. amylovora, Escherichia coli, and Serratia marcescens suggests that the structure of the lipoprotein has been highly conserved under the constraint of efficient gene expression selecting promoter structure, mRNA secondary structure, and codon usage in addition to the polypeptide function. The sequence also suggests that the lpp gene of the three bacteria diverged sequentially in the course of evolution.
测定了包含完整的解淀粉欧文氏菌脂蛋白基因的一段816个碱基对的DNA序列。解淀粉欧文氏菌、大肠杆菌和粘质沙雷氏菌之间的序列比较表明,除了多肽功能外,在高效基因表达对启动子结构、mRNA二级结构和密码子使用的限制下,脂蛋白的结构一直高度保守。该序列还表明,这三种细菌的lpp基因在进化过程中是依次分化的。